Study On The Relationship Between High Risk Human Papillomavirus Infection Merging Different Cervical Lesions And Peripheral Blood T Cell Subsets

Object：To explore the relationship between high risk human papillomavirus infection Merging differentcervical lesions and the expression characteristics of peripheral blood T cell subsets, Research on thedistribution of HPV viral load, age, the course ofdisease in different levels of cervical lesions,To explorethe change of peripheral blood T lymphocyte subsets in the preoperative and postoperative3months ofcervical cancer.Methods: Choose to a total of90patients with cervical lesions and normal control group30cases foundin the cervical cancer screening, and detect HPV-DNA, and do cervical biopsy under colposcope, thehistopathological results was used for a diagnostic standard of final, collect the peripheral blood of patientsfor flow cytometry instrument detection of T lymphocyte subsets.Results:1. Different level of cervical lesions groups were statistically significant in age, course of thedifferences (P <0.05).2. HR-HPV viral load in the chronic cervicitis CIN1+and CIN2~3more was from100to1000, and HR-HPV viral load in the cervical cancer group more than1000, with differencessignificance between different groups(Z=21.795, P <21.795). Different age groups and different levels ofHR HPV had been found two peak loads, respectively for women in reproductive years (<35years of age),perimenopausal women (40to50years old), the difference was statistically significant (X2=6.56, P=6.56<0.05).3. The expression of T cell subsets in peripheral blood in HR-HPV positive mergeringcervical lesions: chronic cervicitis+CIN1group, CIN2~3groups and cervical cancer patients’ peripheralblood CD3, CD4, CD4/CD8ratio were all low than the normal cervix group, while CD8relatively normalgroup increased obviously, and the ratio of CD4to CD8in cervical cancer group even appeared inversion,and the difference of CD4decrease in different groups was statistically significant (P <0.05). Theclassification comparison of CD4/CD8in the different cervical lesions:When the immune function waslow, risk of mild and severe cervical intraepithelial neoplasia and cervical carcinoma in case group was5.47times higher than that of control group (OR=5.47,95%CI (OR)=1.64~15.29). When the immunefunction was disordered, risk of mild and severe cervical intraepithelial neoplasia and cervical carcinoma incase group was1.95times higher than that of control group (OR=1.95,95%CI (OR)=1.58~6.45).4.Themedian of HR HPV viral load in cervical cancer patients preoperative and postoperative3months was1123.68,0.97,the difference was statistically significant (Z=12.87, P=12.87<0.05). CD3, CD4inpatients with cervical cancer in3months postoperatively was high than preoperative, CD8was lower thanthe preoperative, CD4/CD8basically recovered to normal level.Conclusion:1. With the increased level of cervical lesions, cervical cancer incidence age was between45to50years old. HR-HPV persistent infection was a major occurrence cause of precancerous lesions ofuterine cervix and cervical cancer; The viral load of HR-HPV was different with the distribution ofdifferent age.2. CD3, CD4, CD4/CD8ratio in different level of cervical lesions were lower than normal,respectively, with the deterioration of the lesion severity, the expression of CD8was relative strength, andCD4/CD8appeared inversion phenomenon. And the body’s low immune function high increased the riskof cervical lesions than immune dysfunction.3. The viral load of HR HPV was decreased obviously incervical cancer before operation, postoperative3months and the basic immune function back to normallevels.