Study On Establishing Animal Model Of Huizhuotanzu Type Atherosclerosis

Objective: study the way to make Huizhuotanzu type atherosclerosis animalmodel.Methods: divide60ApoE gene knock out mouse into4groups: high fat diet andcold dry environment intervention animal model group, high fat diet control group, colddry environment intervention control group and normal control group.12weeks afternurturing in different groups, the weight, emotion, defecation, tongue presentations wereobserved in all animals. Biochemical indicators and cell morphology were comparedbetween groups after sacrificing all the animals.Results:1: Indicators of Huizhuotanzutype atherosclerosis in high fat diet and cold dry environment intervention animal modelgroup animals are significantly higher than other groups (P<0.05).2. Light microscopicobservations revealed that all the animals developed atheromatous plaque. high fat dietand cold dry environment intervention alone has significantly different affect on thedevelopment of atheromatous plaque comparing to a combined intervention of both highfat diet and cold dry environment (ANOVA: P<0.05). Conclusions: animal underwentcombined intervention of high fat diet and cold dry environment significant biologicalcharacteristics of Huizhuotanzu type atherosclerosis, indicating that animal models weresuccessfully made.