Spectroscopy Detection Of Antimony And Acrylamide In The Environment

It is well known that antimony (Sb) and acrylamide (AM) are both potentiallyharmful to human health. Sb has unknown biological role, but it is a potent toxin.People are exposed to antimony primarily through food and drinking water.Sb maycause adverse health effects such as inflammation of the lungs, chronic bronchitis, andincreased risk of cancer. AM, with a high water solubility, is an α, β-unsaturatedcarbonyl compound, which can be easily absorbed by human body following oral,inhalation and dermal exposure. AM has been shown to cause peripheral nervedamage. Furthermore, long-term intake of AM can induce a number of human geneticdamage and increase the risk of lung cancer. Therefore, it is extremely important todevelop novel methods for determination of Sb and AM in water and food.In the chapter2, a novel fluorescent-based method has been developed for thedetermination of antimony in water. In present study, the spectral characteristic andreaction conditions of Salicyl fluorine-Sb (III) complex were thoroughly investigated.Briefly, in hydrochloric acid medium, a significant decrease in fluorescence intensityof Salicyl fluorine was observed when a certain mounts of Sb (III) were added to thesolution of Salicyl fluorine. Under optimum conditions, there was a linear associationbetween decrease in relative fluorescence intensity (ΔF) of Salicyl fluorine at thewavelength of520nm and the concentration of Sb (III), and the regression coefficientis0.9991. Additionally, the limits of detection for antimony was2.4ng/ml. The realwater samples were then analyzed. The relative standard deviations and the recoverieswere in the range of2.5%～3.1%and96.0%～105.0%respectively. Accordingly, theestablished method was successfully applied to the determination of Sb (III) in watersamples. Moreover, the method was simple and highly sensitive.In the chapter3, we have presented a sensitive HPLC-fluorometric method fordetermination of trace Sb. The sample substances were separated by AgilentTC-C18chromatographic column(5μm,4.6mm×150mm) containing the70%ethanol aqueoussolution. The pump was set at a flow rate of1.0ml/min and the column temperaturewas controlled under30oC. As for the fluorescence detector, the optimal excitation and emission wavelength was494nm and520nm, respectively. The standard curvesshowed linearity over the selected concentration ranges (0.005～0.1μg/ml). Therelative standard deviations for determination of antimony in water were0.9%～1.5%(n=6) and the recoveries were in the range of98.6%～107.0%. The method can beused for practical water sample analysis.In the chapter4, we have proposed a method for the determination of acrylamidein instant coffee by high performance liquid chromatography. The UV wavelength of210nm was chose by diode array detector. An AgilentTCC18column within amethanol-water solution were used in this method. Under the optimized experimentalconditions, the calibration graph was linear for acrylamide concentrations of0.11~100μg/ml. The regression coefficient is0.9999. The detection limit of thismethod was0.06μg/ml. The recoveries of97.60%～104.50%and the relative standarddeviations of0.29%～0.50%were obtained respectively, The method has the distinctadvantage in simplicity, and precision and sensitivity. In total, it is suitable for thedetermination of acrylamide in the instant coffee.