The Expression Of Snail And EMT Protein In Metastatic Spinal Tumor

Objective:The main purpose of this experiment is to research the relationship betweenSnail、E-cadherin、N-cadherin and Vimentin in different Clinicopathological of themetastatic spinal tumor tissue,and explore the role of E-Cadherin, N-Cadherin, Snailand Vimentin in metastatic spinal tumor invasion, metastasis and its value in clinicaldiagnosis, to provide new ideas and methods for clinical prevention of tumormetastasis.Method:Then randomly select60patients`cases of metastatic spinal tumor and20patients`cases of normail persons between2009.1and2012.9. Collect the Clinicaldata(gender,age,Imaging data, Treatment programs)and more. By using the mothod ofimmunohistochemistry SP.We get the expression of Snail/E-Cadherin/N-Cadherin/Vimentin protein in the group of metastatic spinal tumor and the group ofnormal person.We use SPSS18.0software to compare the data of two groups whichstatistical methods use X~2,P value is less than0.05said there were significantdifferences.Results：1. SnailIn the60cases of metastatic spinal tumors,47of them present positive stainingexpression (78.33%); in the20control groups,9groups present positive stainingexpression (45%). The results explain that Snail clearly expresses more than normaltissue in metastatic spinal tumors. Comparing to control group, metastatic spinaltumors are representing significant differences (X~2=7.94, P <0.05).According to Tomita scores, Comparing A+B groups with C+D groups of these60cases of metastatic spinal tumor, There are respectively24cases (66.67%) and23cases (95.83%) positive Snail expression out of36cases (A+B groups) and24case (C+D groups). The difference is clearly significant (X~2=7.88, P <0.01). In these60cases, there was no significant difference (X~2=0.01, P>0.01) between cases withage of younger than60and cases with age of older than60.2. E-CadherinIn the60cases of metastatic spinal tumors,38of them present positivestaining expression (63.33%); in the20control groups,19groups present positivestaining expression (95%). The results explain that E-Cadherin clearly expresses morethan normal tissue in metastatic spinal tumors. Comparing to control group,metastatic spinal tumors are representing significant differences (X~2=7.3429, P <0.01).According to Tomita scores,Comparing A+B groups with C+D groups of these60cases of metastatic spinal tumor, There are respectively30cases (83.33%) and8cases (33.33%) positive E-Cadherin expression out of36cases (A+B groups) and24case (C+D groups). The difference is clearly significant (X~2=7.22, P <0.01). Inthese60cases, there was no significant difference (X~2=0.01, P>0.01) between caseswith age of younger than60and cases with age of older than60.3. N-CadherinIn the60cases of metastatic spinal tumors,42of them present positive stainingexpression (70%); in the20control groups,3groups present positive stainingexpression (15%). The results explain that Snail clearly expresses more than normaltissue in metastatic spinal tumors. Comparing to control group, metastatic spinaltumors are representing significant differences (X~2=18.44, P <0.01).According to Tomita scores, Comparing A+B groups with C+D groups of these60cases of metastatic spinal tumor, There are respectively26cases (72.2%) and16cases (66.67%) positive N-Cadherin expression out of36cases (A+B groups) and24case (C+D groups). The difference is not clearly significant (X~2=0.21, P>0.05). Inthese60cases, there was no significant difference (X~2=0.01, P>0.01) betweencases with age of younger than60and cases with age of older than60.4. VimentinIn the60cases of metastatic spinal tumors,51of them present positive stainingexpression (85%); in the20control groups,7groups present positive stainingexpression (35%). The results explain that Snail clearly expresses more than normaltissue in metastatic spinal tumors. Comparing to control group, metastatic spinaltumors are representing significant differences (X~2=18.81, P <0.01). According to Tomita scores, Comparing A+B groups with C+D groups of these60cases of metastatic spinal tumor, There are respectively29cases (80.56%) and22cases (91.67%) positive Vimentin expression out of36cases (A+B groups) and24case (C+D groups). The difference is not clearly significant (X~2=0.66, P>0.05). Inthese60cases, there was no significant difference (X~2=0.01, P>0.01) between caseswith age of younger than60and cases with age of older than60.Conclusion:1. The research found that the expression of Snail，N-Cadherin and Vimentin haveincreased in the metastatic spinal tumors,the expression of E-Cadherion havereduced.2. Snail and E-Cadherin have been associated with the patients`Tomita score,theexpression of Snail,E-Cadherin,N-Cadherin and Vimentin have not beenassociated with the age of the patients.3. It shows Snail,E-Cadherin,N-Cadherin and Vimentin close correlation withmetastatic spinal tumors.