The Regulatory Role Of P38/Fas/Fasl On The Rosiglitazone-induced Apoptosis In Human Gastric Cancer Cell Line MGC7901

Objectives: To investigate the effects of peroxisome proliferator-activatedreceptor gamma(PPARγ) ligand rosiglitazone(ROS) on apoptosis in human gastriclower-differentiation adenocarcinoma cell line SGC7901, and the regulatory role ofp38/Fas/Fasl on the rosiglitazone-induced apoptosis in MGC7901.Methods: First, gastric SGC7901cells were cultured in vitro, cells ofexponential phase of growth are used to experiment. Using different concentrations ofROS role in SGC7901,48hours later, flow cytometry method was used to detect thecell apoptosis, the expression of mRNA of p38, Fas, Fasl was tested by RT-PCR, theexpression of protein of p-p38, Fas, Fasl was checked by Western-Blot assay.Results: ROS could promote the apoptosis of gastric cell line SGC7901in adose-dependent manner. Flow cytometry method showed that, ROS (25μmol/l,50μmol/l,100μmol/l) role in SGC7901for48hours, the apoptosis rates of the threegroups were26.67％±4.50％,53.67％±3.51％,72.33％±3.06％, the apoptosis of theexperimental groups were significantly higher than the control group (P<0.05).Different concentrations of ROS promoted the expression of mRNA of p38, Fas, Fasland protein of p-p38, Fas, Fasl in SGC7901cells(P<0.05).Conclusions: ROS can promote the apoptosis of gastric cell line SGC7901in a dose-dependent manner.One of the possible mechanisms of apoptosis is that ROScan improve the expression of p38or the phosphorylation level of p38, then increasethe expression of Fas/Fasl, finally promote the apoptosis of SGC7901cells.