Expression Of BiP And CHOP In White Blood Cells From Polycystic Ovary Syndrom

Polycystic ovary syndrome (PCOS) is the commonest endocrine abnormality diseasesoccurring in female and a major cause of ovulation disorder infertility. PCOScharacterized by chronic anovulation, hyperandrogenism, or hyperinsulinism andinsulin resistance(IR).The exact incidence and pathogenesis of PCOS is still unclear. IRmay be the pathological mechanism of PCOS. IR cause hyperinsulinism andhyperandrogenism, resulting in anovulation, sugar and fat metabolism disorder, leadingto long-term complications such as diabetes, high cholesterol and cardiovascular diseaseat increased risk. Studies have shown that endoplasmic reticulum stress (ERS) cause IR.IR is a mechanisms of chronic metabolic disease. So we guess that ERS may lead to IRof PCOS patients. ERS is a state of the pathology of organelles and a self-defencemechanism, too. ERS is due to some reasons lead to endoplasmic reticulum cellphysiological function disorder, calcium dyshomeostasis and misfolded or unfoldedproteins gathered in the endoplasmic reticulum lumen. In view of BiP and CHOP are thetwo symbolic proteins of ERS. In the present study, we primarily observe whether theexpression levels of BiP and CHOP are altered in white blood cells from PCOS by usingRT-PCR and Weston blotting methods, in order to investigate whether ERS is involvedin the pathogenesis of PCOS.Objective:The purpose of this study is to know the expression levels of BiP and CHOP in whiteblood cells from PCOS. Based on the experiment, we try to make further exploration onthe pathogenesis of PCOS. Methods:We took44cases of white blood cells from PCOS, women with PCOS were selectedaccording to Rotterdam Criteria. And50control patients had to fulfill the followingselection criteria: aged<35years and regular menstrual cycles,besides,the diagnosisincluded male factor or tubal factor infertility. PCOS patients were divided into twogroups on the basis of HOMA insulin resistance index(when HOMA-IR>2.69, thepatient existed IR). The expression levels of BiP and CHOP were detected by RT-PCRand Western-Blotting.Results:1.Comparison of clinical indicators of the two groupsThere were no significant differences in age, BMI, PRL, FT3, FRT4, TSH, TCH, LDL,APOB and LPA between PCOS patients and control patients (P>0.05). However, A2,LH, E2, T, FTI, GLU, INS, HOMA-IR, TG and VLDL were higher in white bloodcells from PCOS patients than from control patients(P<0.05). Moreover, SHBG, FSH,HDL and APOA were lower in white blood cells from PCOS patients than fromcontrol patients(P<0.05).2. Expression levels of BiP and CHOP mRNA in white blood cells between PCOSgroup and control groupLevel of BiP mRNA was significantly higher in white blood cells from PCOS patientsthan from control patients(1.67±1.02VS1.04±0.54, P <0.05). Level of CHOP mRNAwas higher in white blood cells from PCOS patients than from control patients(0.79±0.49VS0.63±0.47), but there was no significant difference (P>0.05).3.Expression levels of BiP and CHOP protein in white blood cells between PCOS groupand control group Level of BiP protein was significantly higher in white blood cells from PCOS patientsthan from control patients(0.76±0.12VS0.65±0.09,P <0.05).Level of CHOP proteinwas higher in white blood cells from PCOS patients than from control patients (0.75±0.30VS0.71±0.25), but there was no significant difference (P>0.05).4.Expression levels of BiP and CHOP mRNA and protein in white blood cells ofPCOS with IR and non-IRThere were no significant differences of levels of BiP and CHOP mRNA in white bloodcells of PCOS in IR patients than in non-IR patients. There also were no significantdifferences of levels of BiP and CHOP protein between the two groups.Conclusion:Abnormal expression levels of symbolic proteins of ERS from PCOS suggest that ERSmay be involved in the pathogenesis of PCOS. But there were no significant differencesof levels of BiP and CHOP mRNA and protein in white blood cells of PCOS in IRpatients than in non-IR patients. So ERS whether or not to be involved in the occurrenceof IR in PCOS patients will have further study.