Preparing And Researching The Related Performance Of Hydroxyapatite/Chitosan/Polylactic Acid As Scaffold Material In Bone Tissue Engineering

Objective Preparing hydroxyapatite/chitosan/poly (lactic acid) ternarynanocomposite scaffold material, detecting its physical properties such as mechanicalproperties, porosity, hydrophilicity and so on; Biocompatibility is detected throughinoculating osteoblasts in the scaffold. Exploring the scaffold material as a preliminarystudy of the bone tissue engineering scaffolds is feasibility.Methods Hydroxyapatite/chitosan/poly(L-lactic acid) ternary nanocompositesscaffold was prepared by in situ method and solution blending method, physicalproperties is detected with measuring the porosity by BET method, observing itsinternal structure by SEM; and the same time testing the degradation rate of scaffoldsand degradation of solution PH value. Separation from the rats calvaria boneosteoblasts,cultured, cryopreserved, experiment. The osteoblasts are cultured into thescaffold materials in vitro, osteoblasts,growth, adhesion and proliferation areobserved in scaffold materials with inverted microscope,scanning electron microscopy(SEM). Osteoblasts in HA/CS/PLLA ternary nanocomposite scaffold is dyed withLiving/dead cells double dye kits. Observing and calculating cell survival situation inthe scaffold by fluorescent inverted microscope. Rate of cell proliferation inHA/CS/PLLA ternary nanocomposite scaffold materials is determined by MTTmethod. Results The scaffold pore size distribution is about100~300microns, void fraction(81.3+1.1)%; Mechanics of compressive strength of160mpa; water contacting angleof62o,which confirm that the support material such as internal structure and physicalproperties is great. HA/CS/PLLA ternary nanocomposite scaffolds in SBF without asudden degradation can still maintain a normal solid form and the change of PH indegradation liquid is not obvious with maintaining at PH=7.4; Osteoblasts inHA/CS/PLLA ternary nanocomposite scaffold materials can normally proliferatethrough the scaffold materials of physical properties,live/dead cell dyeing experiments,as well as determined by MTT experiment.Conclusion HA/CS/PLLA ternary nano composite scaffold is confirmed by a seriesof experiments in vitro together with porosity, aperture and mechanical properties,water contact angle, the advantages of good biocompatibility, controlled degradationrate that is for furtherapplication and clinical development of bone tissue engineeringscaffold material of guiding significance.