Basic Research Of Constructing Guinea Pigs Cochlear Biological Three Dimensional Numerical Model

The cochlear is the peripheral sensors of hearing system, depending on thevibration of the basement membrane and endolymph flowing to realize its function. Thecochlear hides in petrous part of temporal bone, its structure is fine and complex,making the physical process difficult to observe and record. For many years, researchersquantitative have analyzed the biomechanics of cochlear and the adjacent building usinganimal and human trials, therefore people are not comprehensive to understand andknow the cochlea. The research of cochlear related diseases/etiology and pathogenesis,diagnosis and treatment methods, the complex interaction with the environment werelimited with lack of accurate quantitative analysis model. Biomechanical modelestablished by the method of combining finite element and experiment model havemade up for the defect. Reconstruction of3d image can not only reappear the tissueorgan of integral space form, but also be based for measuring data of the anatomicalstructure of the cochlea, providing the theory basis for operation positioning, moreoverquantitatively analyzing the cochlea physiological functions. The accuracy of the modelcould be affected by the data that came from the references. It is rare to construct threedimensional numerical model organisms thr of this field. In this experiment, originaldata were obtained according to froough acquisiting digital information of cochlearmacro and mesoscopic anatomical structure, therefore directly impact on puttingforward the original scientific research achievementszen section of the guinea pigcochlea, thencochlear3d dimensional numerical model was established, and thenumerical simulation results of the basement membrane wasdiscussed.Purpose:1.To explore the method of cochlear3d reconstruction by using different serialsection of the cochlea model for establishment.2.According to obtaining the original data of the guinea pig cochlea, cochlear3d dimensional numerical model was established. This paper Mainly obtained original dataof a share of the cochlea basement membrane.3.To discusses the dynamic characteristics of the cochlea basement membrane andstructural characteristics by analysing modal and harmonic response on the basementmembrane of the cochlea.4.To find the influence factors of the basement membrance vibration model andnatural frequency by establishing several differents basement membrane model.Methods:1.Took frozen section of the guinea pig ear,dying and sealing section.2.Obtained a whole set of enlarged image data of guinea pig’s inner ear tissuessections by shooting the sections.3.Treated the images with Photoshop and obtained the3-dimensional coordinateof the boundary by MATLAB software.4.Established3-dimensional model of cochlea and simplified model of cochlearmembrane by using ANSYS software.5.Used the finite element method to modal analyze and harmonious responseanalyze simplified basement membrane model.6.Solved and compared the natural frequency of the basement membrane byseveral different parameters of the basement membrane model.Results:1.Obtained a whole set of guinea pigs cochlear tissues sections and enlargedimage data using quick freezing and shooting.2.digital information of cochlear macro and mesoscopic anatomical structure wereacquisited through histotomy, Constructed the guinea pig Cochlear film lost3dgeometric model by ANSYS software.3.The original data of part of the basement membrane were obtained according tothe constructed Cochlear three dimensional numerical model, the average: the length ofthe basement membrane near the bottom of the cochlea was0.05mm, the length of thebasement membrane near the top cochlear was0.25mm, the length of the basementmembrane near the modiolus was1.2187cm, the length of the basement membranefaraway from the modiolus was1.4969cm.4.Obtained ten order before the inherent frequency of the basement membrane byusing the finite element method to modal analyze and harmonious response analyzesimplified basement membrane model（3189.9Hz—5897.9Hz）. 5.The basement membrane model precision was positively correlated with thebasement membrane of the screw surface level of distortion, the thickness and its ratio.Conclusion:1.3d reconstruction of the inner ear minute structure by frozen serial sections andcomputer technique is a method worthy to be discussed.2.Dynamic properties of basement membrane was numerical simulated andanalyzed using biomechanical model that constructed by the experiment model andfinite element method, comparing the results was compared with the data from relevantdocuments, proving the feasibility of the model to simulated cochlear feeling note andthe basement membrane value.3.The shape, thickness and thickness ratio of the basement membrane were foundto be very sensitive to the analysis of the natural frequency value of the basementmembrane. Numerical simulation results was positively correlated with the basementmembrane of the screw surface level of distortion, providing basis for furtherestablishing numerical model of cochlear biological fine.