Effects Of Fluorine-ion Implantation Titanium Surface Modification On The Interaction Of Osteoblast And Porphyromonas Gingialis

Objective: Currently, researchers begin to carry out studies on the inhibitingeffects of fluoride to gram negative bacteria to achieve the goal of preventingperiodontitis and peri-implantitis or peri-implantoclasia. In our study, we applied theplasma immersion ion implantation technique to implant fluoride ion into the smoothsurfaces of pure titanium. The aim of our present study was to evaluate the physicalstructure and chemical composition of the modified surfaces as well as assess the effecton the biocompatibility and antibacterial activites, which were pivotal in evaluating theviability of a material used for implanting into human body.Methods:1. Fluoride ion implantation and microscopical analysisThe test specimens in the form of discs10mm in diameter and1mm thick wereground down and polished one side as a mirror finish and then they were sequentiallyultrasonicated in acetone, absolute alcohol and deionized water, air-dried and stored in adesiccator. Prior to plasma immersion ion implantation processing, the samples weresputtered by argon plasma for10minutes.2、Effects of Fluorine-ion implantation titanium surface modification on theinteraction of osteoblast and porphyromonas gingialisMG-63cells were cultured in96-well cell culture plates at a density of5,000cells/ml. After the adherence of the cells, the α-MEM medium was replaced by theP.g leaching liquor of the DMEM medium. The effects on the generation of MG-63were examined by CCK-8、ALP and real-time PCR.Results:1. Gross observation: A total of36implants, A group has2implants. They weretaken out because of loosening at2th surgery. The other implants have no clinical mobility, percussion sound crisp and no postoperative inflammation. Success rate ofplanting: A group83.33%, B group100%, C group100%.2. Effects of Fluorine-ion implantation titanium surface modification on theinteraction of osteoblast and porphyromonas gingialisFluorine ion implantation group on the surface of titanium in gum porphyrin formafterbirth fungus under the action of osteoblestlike cells MG-63cell proliferationnumber significantly more than the control group pure titanium surface, with significantdifference (P <0.05)All material surface P.g in under the action of MG-63cell activity training withthe extension of time and reduce, Fluorine ion implantation pure titanium surface cellsof protein activity than not injection of pure titanium group high, with significantdifference (P <0.05)Four groups material surface OPG and RANKL mRNA expression are allpositive, sterile cases fluorine ion implantation material surface of osteoblast secretecollagen type OPG and RANKL mRNA quantity and pure titanium material surface noobvious difference.But in gum porphyrin form afterbirth fungus under the action of fluorineion implantation group of collagen type material surface OPG and RANKL mRNA expressa pure titanium group of surface, a statistically significant difference (P <0.05)Conclusions:1. The chemical elements of titanium were Titanium, Oxygen and Carbon; Thechemical elements of fluoride ion-implanted titanium were Titanium, Oxygen, Fluorineand Carbon.2. Fluorine ion implantation pure titanium material surface modification in gumporphyrin form afterbirth fungus under the action of osteoblast quantity is pure titaniummaterial surface, cells more toxic pure titanium surface.3. Fluorine ion implantation pure titanium material surface modification in gumporphyrin form afterbirth fungus under the action of the osteoblast protein activity is apure titanium material surface high, more conducive to bone cell growth.4. Fluorine ion implantation group material surface material the osteoblast OPGand RANKL mRNA collagen type of expression to have higher than pure titaniumgroup the trend of the surface.