Impact Of Exposure To X-ray On Adhesion Of Endometrium With Embryo

Objective: X-ray is a kind of common ray in daily life. X-ray is also a kindionizing radiation. It has been proved the penetrability of X-ray is much more powerful,so it is harmful to biology to lead to pathological damage and dysfunction. The reportshows that in the first week after conception, the X-ray damage to these cells willprobably result in failure to implant or undetectable death, especially in the organsunder development at the time of exposure. Besides, malformations might occur at thesame period. In these years, the frequency of infertilitas feminis is getting higher, evenget a high proportion around10-15%. But there is no specific proof to show that thesecases due to contact with the ionizing radiation product. Therefore study the embryos ofX-ray has a certain necessity.This experiment is to use X-ray to treat endometrial cell with the detection of p53and its downstream target proteins. And we analyze the embryonic cell adhesionfunction after X-ray exposure with vitro embryo adhesion model to explore theembryonic cell adhesion molecules mechanism with the impact of X-ray.Methods:(1) Culture the high-receptive RL95-2cells. Use Single Cell GelElectrophoresis (SCGE) to detect the breakdown of DNA strands in RL95-2withexposure of different does of X-ray (1Gy or10Gy).(2) Use Western Blot to detect p53and its downstream signaling pathway protein’s expression in RL95-2after X-rayradiation.(3) Prepare RL95-2monolayer with the treatment of different dose of X-ray.Add embryonic JAR cells on it to set up the embryo adhesion in vitro model. Analyzethe percentage of embryo adhesion in the control and X-ray treated groups.Results:(1) The effect of X-ray-induced DNA damage was presented in SCGEtest. It was found that RL95-2cells treated with X-ray (1Gy and10Gy, respectively)resulted in a significant increase in the number of DNA strand break.(2) Western Blotresults showed a significant accumulation of p53in RL95-2cells treated with X-ray. The protein content in high dose group is lower than that in low dose group, while p21and p27had no obvious change.(3) The percentage of adhesion in high dose X-raygroup was lower than that in the low dose group.Conclusion: Different dose of X-ray all does impact on the intercellular adhesionbetween endometrial cells and embryonic cells. The X-ray-induced DNA strand breaksdamage the adhesion embryo to RL95-2cell monolayer. In the case of DNA strandbreaks, p53has a massive accumulation. It suggests that p53has a certain relationshipwith endometrial cell adhesion function. The accumulation of p53may lead to thereduction of adhesive function of endometrial cells. The study suggests that the X-rayradiation-induced DNA damage can impair the receptivity of RL-95cells to embryo. Itsupply the partial data related to the X-ray radiation and infertility.