Effect Of Salidroside On Proliferation And Anti-apoptotic Mechanism Of Bone Marrow Mesenchymal Stem Cells

Objective To investigate the effect of salidroside on human bone marrowstromal cells (hBMSCs) proliferation，and the apoptosis of hBMSCs induced bycytarabine (Ara-c).Methods hBMSCs were isolated by Fircoll density gradient centrifugation;Morphologic appearance of hBMSCs were observed under an optical microscope;Cell surface antigens were measured by flow cytometry; The osteogenic andadipogenic differentiation was tested and evaluated by specific staining methods; andthen MTT examinate cell multiplication situation,. The apoptosis of hBMSCs weredetected by flow cytometry. The expression of SCF、 Bcl-2、Bax mRNA was assayedby RT-PCR.Results The adherent cells showed spindle shape and fibroblast cell-likeshape;The expressions of CD44、CD71and HLA-ABC were positive, the expressionsof CD34、CD45、HLA-DR in hBMSCs were negative; The cells were successfullyinduced into osteogenic and adipogenic lineages, these cells were showedmineralization presented with von kossa staining. Furthermore, liquid vacuoles weredetected by oil red O staining; There were significant suppression of hBMSCs afterAra-c treated, slidroside promoted the proliferation of hBMSCs, and apoptosis ofhBMSCs was significantly lower with slidroside pretreatment; The expression levelsof SCF、BCL-2mRNA in salidroside pretreatment group were up-regulated, BAX mRNA were low-regulated.Conclusions Salidroside can promote the proliferation of hBMSCs and increaseSCF secretion in vitro；and it can inhibit the apoptosis of hBMSCs induced by Ara-c,The mechanism might be mediated the expression BCL-2/BAX.