The Influences On Proliferation, Migration, Invasion, And Drug Resistance Of NPC Cell Line CNE-2by SATB1Silencing

Objective To investigate the role of special AT rich sequence binding protein1(SATB1) in proliferation, migration, invasion and drug resistance of nasopharyngealcarcinoma(NPC) cell line CNE-2.Methods To detect the location of SATB1expression in CNE-2using cellimmunofluorescence; the recombined shRNA plasmid that targeted SATB1wasconstructed and transected into the CNE-2cell to silence SATB1expression; theSATB1mRNA and protein expression in CNE-2cell was examined by RT-PCR andwestern blot. The proliferation ability and drug resistance of cells were examined byCCK8method; the invasion ability of cells was examined by transwell assay; themigration ability of cells was examined by wound healing test.Results1. Cell immunofluorescence revealed that SATB1was localized in thenuclei;2. In transwell assay, the transmembrane numbers of parental CNE-2, controlshRNA and SATB1-shRNA were58.33±7.64,34.67±5.03,57.33±2.52,respectively, compared with control and parental group, the transmembrane number ofSATB1-shRNA was reduced, the difference was significant(P<0．05), however thedifference between control and parental group was not significant(P>0.05);3. Inwound healing test, the migration distance of parental CNE-2, control shRNA andSATB1-shRNA were95.42±7.37,89.89±16.57,50.97±17.72, respectively,compared with control and parental group, the migration distance of SATB1-shRNAwas reduced, the difference was significant(P<0.05), however the difference betweencontrol and parental group was not significant(P>0.05);4. In the cell proliferationassay, the cells of SATB1-shRNA group grow faster than that of control shRNA andpratental CNE-2group, the difference is significant(P<0.05);5. In the drug resistanceassay, the inhibition rates of parental CNE-2, control shRNA and SATB1-shRNAwere0.50±0.03,0.51±0.03,0.57±0.02, respectively, compared with control andparental group, the inhibition rate of SATB1-shRNA was reduced, the difference was significant(P<0.05), however the difference between control and parental group wasnot significant(P>0.05).Conlusions Down-regulatation of SATB1by shRNA could inhibit the growth,invasion, migration and drug resistance of CNE-2.