Expression Of The Chemokine And Its Receptor In Peripheral Blood Of Severely Scalded Rats And Its Relationship With Inflammatory Mediators

Objective:To investigate the expression of the chemokine and its receptor in peripheralblood of severely scalded rats and its relationship with inflammatory mediators, andto analyse its mechanism.Method:1. According to the randomly number table, forty-two male SD rats were divided intothe scald group(n=36)and the control group(n=6). The rats in the scald group weresubjected to30%full-thickness burn on the dorsal skin, while rats in the normalcontrol group were sham scalded with37℃water. Six rats in the scald group weresacrificed at post injury hour (PIH)1,3,6,12,24and48to separate erythrocyte,mononuclear cells and plasma from peripheral blood.2. The expression of the erythrocyte DARC protein was determined by Western Blot.The levels of chemokine IL-8, MCP-1and RANTES in erythrocyte and plasma weredetected by enzyme-linked immunoadsordent assay (ELISA), and the levels ofTNF-α, IL-1β and IL-6in plasma were measured by ELISA.3. The expression of84genes that encode chemokines and their receptors wereprofiled by the Chemokines and Receptors RT2Profiler PCR Array, which containsmembers of the C-C and C-X-C motif subfamilies of small inducible cytokines andtheir receptors as well as other related genes.Result:1. Compared with the control group, the expression of the erythrocyte DARC proteinwas significantly down-regulated in the scald group (P<0.05); the levels of theerythrocyte IL-8were obviously increased at PIH1and PIH48(P<0.01), but these were significantly decreased (P<0.05) from PIH3to PIH24; the levels of theerythrocyte MCP-1were decreased after PIH1, which were statistically significantdifference at PIH3(P<0.05)，however, there were significantly increased at PIH48(P<0.05); the levels of the erythrocyte RANTES were distinctly decreased afterPIH1（P<0.05－0.01）.2. Compared with the control group, the levels of plasma MCP-1and IL-8wereobviously increased after PIH1（P<0.05－0.01）; the levels of plasma RANTES wereincreased from PIH1to PIH12, and there were statistically significant difference atPIH1and PIH3（P<0.01）.3. Compared with the control group, the levels of plasma TNF-α and IL-1β weresignificantly increased after PIH1（P<0.05－0.01）; the levels of plasma IL-6wereincreased after PIH1, and there were statistically significant difference at PIH1, PIH3and PIH6（P<0.01）.4. Compared with the control group,22of84genes, which contains members of theC-C and C-X-C motif subfamilies of small inducible cytokines and their receptors aswell as other related genes., were statistically significant difference in the scald group（P<0.05－0.01）.Conclusion:1. The expression of the DARC of erythrocyte was reduced in rats after the scald,which might be a reason of chemokine and related inflammatory mediatorup-regulated in the scald rats, ant it suggested the DARC of erythrocyte could involvein the inflammatory response after burn through regulating chemokine levels.2. PCR chip technology could provide rapidly and accurately the difference geneexpression profiles of chemokines and their receptor of the mononuclear cells in thescald rats, which provide a new way to study inflammatory signaling pathways andthe clinical immunotherapy after scald.