| Cancer is a major public health problem in many parts of the world with a lot offactors involved and complex process. It has been proven in numerous studies thatchemical mixture exposures and viral infections are two major risk factors of cancer.However, most researches of cancer in etiology have been mainly on single factorpresently. For lack of effective, quick and feasible methods in cancer risk assessmentof joint effort, the relationship between chemical-chemical/chemical-gene combinedeffect and the cancer is hard to identify quantitatively, and no conclusion has beenreached so far.The Salmonella mutagenicity test, specifcally designed to detect chemicallyinduced mutagenesis, was adopted to detect the mutagenicity of alone chemical orchemical mixtures. Mutagenic test results were found in different doses ofMethylnitrosourea, Diethylnitrosamine and3-Methylcholanthrene respectively. Theresults also showed that2μg per plate Cr (Ⅵ) and5μg per plate Nimustine alone toTA98were negative, whereas, positive results turned out when they mixed at the samedose. Similar condition was found in Ames assay of TA100.1μg per plate Cr (Ⅵ) and1μg per plate Nimustine didn’t show up mutagenicity independently. However, clearpositive results were obtained when they joined together. UV-visiblespectrophotometry was adopted to detect the toxic doses of chemicals and the resultswere consistent with the Ames assay. On the other hand, we took the normal humanembryonic liver cell CCC-HEL-1as the effector cell and transfected the x gene ofHepatitis B virus which is inextricable with liver cancer. After screening theCCC-HEL-1-HBx cell line with stable expression in the future, the malignanttransformation system based on normal human cell could be applied to assess the jointeffect of HBV-x and chemicals. This investigation threw light upon the detectionmethod of combined effect from chemical-chemical to chemical-virus and willprovide new system to recognize the carcinogenic risk of multi-factor model. |
PDF Full Text Request