The Study On Prevention Of Paclitaxel Associated With All-trans Retinoic Acid On Allograft Angiopathy In Rats

Obiective：To investigate the prevention of Paclitaxel and At-RA（All-trans retinoicacid）respectively and their associated effect on allograft angiopathy in rats, anddiscuss their potential mechaniam of action and possible synergistic action．Methods: The establishment of allograft model was used with carotid transplantationin rats.60rats were randomly classified into four groups：the control group(A)，At-RA–treated group (B)，Paclitaxel-treated group (C) and combination-treatedgroup(D)．At0,1,4,8weeks after transplantation, the lipoprotein profile and NOcontent、ALT、Crin blood serum were detected, also the grafts were harvested toobserved the regional changes in the intimal layers by light microscopy and theexpression of TNF-α、PCNA、CD4+T cells in allografts by immunohistochemistry．Results: There was the least intimal thickness, the highest level of NO in blood serum,the least expression of PCNA、TNF-α and the expression of CD4+T cells in groupD．The opposite is group A. And group B and C are better than groupA．(P<O．05)Conclusion: The prevention of Paclitaxel and At-RA on allograft angiopathy wassignificant at the dose of this experiment,and the effect was most obvious in acombination of both drugs．At-RA may exert its function to prevent allograftangiopathy by the mechanism of secretion of NO，indirect anti-inflammatory effect,commissural inhibition on the expresstion of TNF-α、PCNA and CD4+T cells and theproliferation of SMC．...