The Significance Of Glyoxalase â…  Level In Serum In Type2Diabetic Macrovascular Complications, And Its Relationship With Oxidative Stress

[Objective]1. To observe the glyoxalase I (GLO Ⅰ) level in serum of patients withType2diabetes and normal control group, investigate the significance of GLO Ⅰ indiabetes and diabetic macrovascular complications.2. To observe the relationship between GLO Ⅰ, advanced glycation end products(AGEs), and oxidative stress targets like malonyldialdehyde (MDA), superoxidedismutase (SOD), glutathione peroxidase (GSH－Px) in patients with Type2diabetes.[Methods] Select34cases with type2diabetic who hospitalized in the Department ofEndocrinology ward of Fujian Medical University Affiliated Union Hospital fromSeptember2012to March2013, including20cases of type2diabetes withmacroangiopathy(DM1group), the average age (57.60±8.05) and14cases of type2diabetes without macroangiopathy(DM2group), the average age (54.93±10.78) yearsold, and22cases who underwent healthy examination and showed no diabetes andtheir complications served as normal control group(NC group)．each patient isdetermined AGEs, GLO Ⅰ in serum with ELISA method and measured clinical datalike the height, weight, fasting blood glucose, glycosylated hemoglobin(HbA1c),triglyceride (TG), cholesterin(CHOL), high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C), High-sensitivity C-reactiveprotein(hs-CRP), homocysteine(Hyc), intima-media thickness(IMT), ankle-brachialIndex (ABI), brachical-ankle artery pulse wave velocity (baPWV),and measuredoxidative stress targets like MDA, SOD, GSH－Px. Compare the different indicatorsbetween each group of patients.[Results]1. The multiple linear regression analysis of GLO Ⅰ, AGEs and groupingdemonstrated: the grouping had a significant impact on GLO Ⅰ (P<0.001) and AGEs(P<0.01), it meant that GLO Ⅰ levels increased with DM1, DM2and NCgrouping sequence, and AGEs levels decreased.2. The relevance analysis of GLO Ⅰ, AGEs and various diabetes targetsdemonstrated: GLO Ⅰ was positively correlated with HDL-C, but negatively correlatedwith serum AGEs level, duration of diabetes, fasting blood glucose, LDL-C, systolicpressure and diastolic pressure. AGEs was positively correlated with TG, fasting bloodglucose, LDL-C, but negatively correlated with GLO ⅠI.3. The PEARSON relevance analysis of GLO Ⅰ, AGEs and vascular targetsdemonstrated: GLO Ⅰ and baPWV were negatively related, AGEs and baPWV werepositively related.4. The relevance analysis of GLO Ⅰ, AGEs and oxidative stress targetsdemonstrated: GLO Ⅰ and SOD, GSH-PX were positively related, AGEs and MDAwere positively related, AGEs and SOD, GSH-Px were negatively related.[Conclusion]1. GLO ⅠI and blood glucose, blood lipid, blood pressure wererelative. In terms of the level of GLO Ⅰ, the normal control group accounted for thegreatest number, followed by the type2diabetes group and the type2diabetes withmacroangiopathy group ranking at the third position. In contrast, the level of AGEssaw an opposite trend. Therefore, GLO Ⅰ may play an important role in the inhibitionof diabetes and diabetic macrovascular complications.2. GLO Ⅰ and baPWV were negatively correlated, prompting the level of GLO Ⅰ may be indicative of diabetic early macrovascular complications.3. GLO Ⅰ and SOD, GSH-PX were positively related, prompting GLO Ⅰ andoxidative stress were closely relative.