Isolation And Identification Of Chicken Escherichia Coli In Jinan And Preparation Of Own Inactivated Vaccine

Escherichia coli disease is caused by some serotypes of Escherichia coli disease conditions, rapiddevelopment in recent years become a major bacterial disease of the poultry industry, causing seriouseconomic losses to the practitioners. During the onset of the disease prevention relies mainly on vaccinesand drugs, but the results are less obvious. This purpose from2008to2012, around Jinan five chicken farmpoultry disease prevalence survey，And chickens from the submission of the disease compound, pathogenisolation and identification of the following major findings:A common disease of chickens according to the nature of the pathogen can be basically divided intothree types of viral, bacterial and parasitic. The survey results show that the viral poultry disease, avianinfluenza incidence and mortality rates are relatively high, followed by Newcastle disease; Bacterialpoultry disease, the incidence of E. coli disease with the highest mortality rate, followed by Staphylococcusaureus disease; Chicken lice and chicken coccidiosis incidence and mortality,2008-2012, are showing adeclining trend. According to the survey results contrast, most of the epidemic disease incidence andmortality are showing a gradual downward trend, especially parasitic diseases has been well controlled,Viral and bacterial diseases remain serious harm to the development of the poultry industry, Especially E.coli disease, bacterial disease often co-infection with other pathogens, morbidity and mortality are high, areon the rise.From Jinan and the surrounding area the typical pericarditis and perihepatitis37the chickens liverdisease compound isolated from E. coli14. Isolates E. coli O-antigen identification, O78, O2, O9, O24, O127,O15, O82seven serotypes. The regional advantages type O78, O2, O9, O24, respectively, accounted for28.6%of the total number of E. coli strains,21.4%,14.2%,14.2%. Isolates susceptibility test results:florfenicol, azithromycin, cefotaxime, Tou Baopai Shu, neomycin, fosfomycin sodium, enrofloxacinsensitive, Will be more sensitive to the drug compound and achieved good results, especially drugsneomycin and florfenicol, azithromycin, cefotaxime, the Tou Baopai Shu complex combination not onlyachieve better results, And greatly reduce drug costs, with good value for money.Many antimicrobial agents can not effectively control E. coli disease of chickens, Therefore,development of a safe and effective seedlings of E. coli is an effective way to control E. coli disease. To the isolated E. coli regional advantages serotypes O78, O2, O9, and O24four advantages serotype strains systemseedlings strain, developed a multivalent inactivated oil adjuvant vaccine, Experimental animal vaccinetesting and a variety of day-old non-immune chicken. The results showed: Escherichia coli developed theirown seedlings, Good stability, The various traits indicators are in line with requirements，Immune to goodeffect. The chicken farm field regression immunoassay, Their own seedlings has good protective effect.