| The present study was conducted to evaluate the effect of different amino acid chelated zinc and iron proportions replacing inorganic zinc and iron in diet on the production performance in broilers; Then learned the effect of different chelated zinc and iron levels on the production performance in broilers basced on the optimum replacing proportion. Finally maked a preliminary study by perfusion test to study the absorption of chelate in duodenum. It can provide experimental data for the scientific application of Amino acid chelated zinc and iron. Three different research were showed as follows:Experiment1:Effects of different proportion amino acid chelated zinc and iron instead of inorganic zinc and iron in diet on the production performance in broilersFour hundred and fifty1-day-old healthy Arbor Acre broilers were randomly allotted into5treatments. Each treatment consisted of three replicates with30broilers. Group1was the control group which was fed basal diet-with100mg/kg inorganic iron and zinc, inorganic zinc and iron was repaced by different proportions of chelated zinc and iron in the other four treatment groups diet.The proportions were25%(experimental group1, inorgnic75mg/kg, chelated25mg/kg),50%(experimental group2, inorgnic50mg/kg, chelated50mg/kg),75%(experimental group3, inorgnic25mg/kg, chelated75mg/kg) and100%(experimental group4, inorgnic0mg/kg, chelated100mg/kg), respectively, the two sampling time were21and42d.The results showed that compared with the control group, average daily gain (4-6WK) of the group3and4were significantly (P<0.05) increased by8.17%and8.07%than the control; the ratio of feed intake to gain (4-6WK,1-6WK) of group4were significantly (P<0.05) decreased; and there was no difference for feed intake among groups (P>0.05)Half net carcass rate and total net carcass rate of treatments were significantly (P<0.05) increased on42day; however, no difference was detected for thigh meat ratio and the abdominal fat ratio among groups (P>0.05) The chest tendernesses of the group4were significantly (P<0.05) decreased; protein in breast and thigh of the group4were significantly (P<0.05) increased; no effect was detected on water,fat and ash in breast and thigh among groups (P>0.05)The serum albumin of the group4were significantly (P<0.05) increased; there was no effect on Total Protein, globulin, urea, urea nitrogen, CHOL and triglyceride in serum (P>0.05)Experiment2:Effects of different chelated zinc and iron levels on the production performance in broilersThree hundred and sixty1-day-old healthy Arbor Acre broilers were randomly allotted into6treatments. Each treatment was consisted of three replicates with20broilers in. The Control Group was fed basic diet with100mg/kg of zinc (ZnSO4) andiron (FeS04.7H2O) content. The Group1to5were added zinc-amino acid chelate (Zn-AA) and iron-amino acid chelate (Fe-AA) at25,50,75,100, and125mg/kg level, the two sampling time were21and42d.The results showed that the weight (21d) of the group2were greater than the control6.78%(P<0.05); the weight (42d) of the group5were greater than the control9.12%(P<0.05)Average feed intake (4-6WK,1-6WK) of the group5were significantly (P<0.05) increased by16.27%and15.33%than the control; average daily gain (4-6WK) of the group5were significantly (P<0.05) increased by13.68%than the control; there was no difference for the ratio of feed intake to gain and mortality among groups (P>0.05)The breast meat ratio of the group2was greater than the control on21day (P<0.05); and that of the group5was significantly increased on day42(P<0.05); no effect was detected on the other parameters of slaughter performance (P>0.05)The breast tendernesses of the group2and4were significantly (P<0.05) increased by22.79%and27.34%than the control; the protein of experimental groups significantly increased (P<0.05), especially group5increased by6.62%than the control.The zinc and iron of the group3and4in liver were higher than control (P<0.05)Experiment3:The absorption characteristics of chelated iron and zinc in duodenum of broilersOne hundred and eighty21-day-old Arbor Acre broilers were selected and randomly allotted into5treatments, Each treatment was consisted of three replicates which was picked6 broilers for slaughter. The Control Group was infused with40μg/mL of zinc (ZnSO4) and iron (FeSO4.7H2O) content in duodenum. Chelated zinc and iron were added to the perfusate of the four treatments with the proportion of25%(experimental group1, inorgnic30μg/mL, chelated10μg/mL),50%(experimental group2, inorgnic20μg/mL, chelated20μg/mL),75%(experimental group3, inorgnic10μg/mL, chelated30μg/mL) and100%(experimental group4,inorgnic0μg/mL,chelated40μg/mL). the five sampling time were0,15,30,60,90and120min after infusion.The results showed that the zinc and iron of experimental groups were more higher than the control on30min (P<0.05); The copper of the group3and4were more higher than the control on60,90and120min (P<0.05)The copper (90,120min) of the group3and4were more higher than the control in blood (P<0.05); and no effect was detected for zinc and iron in blood among different sampling time (P>0.05)... |
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