The Research On Injury Mechanism Of Deoxynivalenol, Zearalenone And Co-infection On Brain Of Mice

Three hundred and sixty healthy female mice were randomly assigned to9groups: control group (10%methanol), DON infected group (test team D1and test team D2, dose of DON were1.5、2.5mg/KgBW)、ZEA infected group (test team Z1and test team Z2, dose of ZEA were20、30mg/KgBW)、combined infected group (D1Z1:DON1.5mg/KgBW and ZEA20mg/kgBW, D1Z2:DON1.5mg/KgBW and ZEA30mg/KgBW, D2Z1:DON2.5mg/KgBW and ZEA20mg/KgBW, D2Z2:DON2.5mg/KgBW and ZEA30mg/KgBW). The test were two factors and three levels:DON concentration levels of0,1.5,2.5mg/KgBW, ZEA levels of0,20,30mg/Kg BW, were injected by peritoneal cavity every24h for4d successively.4mice per group were killed by cutting neck and cerebrum samples were immediately harvested at different time points (morning of3d,5d,8d, and12d). Weight of the brain tissues were recorded and stored in liquid nitrogen containers. Protein contents, antioxidant indicators and neurotransmitter quantities as well as apoptosis rate were determined.The results were as follows:(1) Wight of the brain samples from each group showed no significant difference during the test (P>0.05).(2) The combined or individual contamination both reduced the protein contents dose-dependently. The combined toxins had remarkable additive effect interaction which is evident huger than the single one’s with the protein contents reduction. After injecting4days (8d), the effect decreased gradually until8d post injection (12d).(3) On3d and5d, ZEA low concentrations,DON and ZEA low concentration combined infections can improve SOD activity in brain tissue,other combined or individual contamination both reduced the SOD activity, malondialdehyde (MDA) content increased, tissue hydroxy free radical activity decreased, nitric oxide (NO) content and activity of nitric oxide synthase (NOS) increased. Compared combined toxins exposure group with single toxin exposure group, the difference was significant or extremely significant (P <0.05or P<0.01), and apparente dose-effect relationship between different concentration groups was showed. Among them, the oxidative damages of brain in the combined high concentration group (DON2.5mg/KgBW+ZEA30mg/KgBW) are the most serious.(4) Neurotransmitter disorder was caused by neurotransmitter and single toxin:AchE activity decreased, Glu level increased, GS concentration increased.(5) The apoptosis rates of the single and combined infected groups increased different from the control group.(6) Toxic effect of DON and ZEA increased to the peak on3d and5d, then obviously decreased on8d and12d, but combined toxins exposure group still showed relatively strong toxicity.These results indicate that DON, ZEA alone and the combined toxins exposed mice all could cause mouse brain tissue protein contents and antioxidant capacity decreased and apoptosis rate increased, eventually, result in brain functions impaired; Deuto-acrylic additive effect was the main interaction effect between DON and ZEA.