The Study On Molecular Identification Of Lymantria Dispar In Different Areas

The gypsy moth (Lymantria dispar Linnaeus) is one of the most serious forestpest in temperate Holarctic regions. According to morphological characters, biologicalcharacteristics and geographical distribution, L.dipar is divided into L. dispar asiaticaVnukovskii, L. dispar dispar(Linnaeus) and L. dispar japonica(Motschulsky). L.dispar asiatica Vnukovskii and L. dispar japonica(Motschulsky) are known asAsia-L.dispar, L. dispar dispar(Linnaeus) is known as Europe-L.dispar. It’s difficultto determine their geographic populations only by morphological characters,especially larvae were intercepted at the port. There are muc h difficulties inidentification and need longer quarantine period. For fast and accurate identificationof these similar species of L.dispar, established several rapid identification methods ofL.dispar and analysed molecular genetic regulation by differences between completemitochondrial sequences.The complete sequence of the gypsy moth L. dispar mitochondrial genome hasbeen determined in present paper. The15,569bp long genome is comprised of13putative proteins,2ribosomal RNAs and22tRNAs. According to comparing ninecomplete mtDNA sequence from different areas, laid a foundation for fast andaccurate identification of these similar species of L.dispar.According to the characters of L.dispar mitochondrial gene sequences, the highmutation rate was found and primers were designed for these sequence. The L.disparsamples that came from different areas were sequenced, edited and connected to setup a comprehensive local L.dispar gene bank. The geographic population of unknownsamples will be determined by sequence homology.According to parts of approximate species mtDNA of Asia-L.dispar,Europe-L.dispar and North America-L.dispar, designed specific primers and probes.For the purpose of quickly accurate detection and identification, established has danceHIV moth of real-time fluorescence PCR detection method through on97differentgeographic sources of China, Russia, Mongolia, Japan, Belgium, Turkey, and UnitedStates. 2Lepidoptera larvae were intercepted in red oak from Belgium, according to themorphologic characters they were identified into gypth moth, L.dispar Linnaeus. Asto detective the genotype of the moth, two genetic marker on mitochondrial gene andchromosomal gene were used, and the larvae turned out to be European gypsy moth,and the samples are hybrids.