Standard Map Of Capillary Electrophoresis For The High Molecular Weight Glutenin Subunitsin Common Wheat

Gluten exists in common wheat endosperm is one of the major storage protein and it ismade of some high molecular weight gluten subunits (HMW-GS), through intermoleculardisulfide bond and other non covalent bonding link with a number of low molecular weightgluten subunits (LMW-GS), and formation of protein polymers, it has a great influence to thewheat flour quality. The most commonly used method in the separation and identification ofwheat high molecular weight gluten subunits is polyacrylamide gel electrophoresis (PAGE) orhigh performance liquid chromatography (HPLC) and so on, but these methods in the actualoperation all have different degrees of limitations in the process, it is not conducive tolarge-scale identification of wheat high molecular weight gluten subunits. Capillaryelectrophoresis (CE) technique is a traditional electrophoresis technology improvement andgradually developed a highly efficient separation of new identification technology, it showesa great superiority in the separation of biological macromolecules such as the protein、 aminoacid and nucleic acids research. To provide technical support to identify the high qualitywheat glutenin subunits, capillary electrophoresis (CE) was used to identify some commonhigh molecular weight glutenin subunits. It could be used to reveal biochemical variationamong Glu-1HMW-glutenin subunits in wheat cultivars. The resolution and repeatability ofcapillary electrophoresis technology is analyzed and standard map of15subunits areconstructed,and sorting the standard of the peak time, compares the capillary electrophoresiswith sds-page. The results are as follows:1. Chinese spring varieties is separated for5times, the electrophoresis results are highlyrepetitive, out of the subunits peak time and the height variation, the peak time standarddeviation is0.0826~0.0987, the relative standard deviation (RSD) is0.11%~0.11%, relativestandard deviation is0.20%on average, that capillary electrophoresis results error is small,high reliability.2.Received a total of15of the most common peak time accurately such as1Ax1、1Dx2、1Dx3、1Dx4、1Dx5、1Bx6、1Bx7、1By8、1By9、1Dy10、1Dy12、1Bx13、1Bx14、1By15、1By16.3.Drawn15out of peak time map, to get each order the peak time of1Dy12、1Dy10、 1By9、1By8、1By16、1By15、1Bx14、1Bx13、1Bx7、1Bx6、1Ax1、1Dx4、1Dx3、1Dx5、1Dx2.4.The peak order of capillary electrophoresis and sds-page is a bit different, use thistechnique is simple and rapid to identify wheat high molecular weight gluten subunits.It isquicker than use sds-page appraisal and the results is more sensitive. able to provide efficienttechnical support of rapid determination of a large number of different species of highmolecular weight gluten subunits.According to the results of this study compared with the conventional sds-page methods,high performance capillary electrophoresis for separating wheat high molecular weight glutensubunits have many superiority such as fast speed, high resolution, low sample consumptionand so on.It has a great application prospect in species identification and quality breeding inthe early generation high quality genetic screening.