The Analysis Of Matsucoccus Matsumurae From Three Geographic Populations In China

The Japanese pine bast scale, Matsucoccus matsumurae (Kuwana)(Hemiptera:Coccoidea:Matsucoccidae) is an important invasive alien species in pine forests of China. However, the classification controversy and the population genetic differentiation of this species have puzzled managers. In this thesis, we selected the three representative geographic populations, Jinhua of Zhejiang province (ZJJH), Qingdao in Shandong Province (SDQD) and Fushun in Liaoning Province (LNFS). We collected samples of M.matsumurae, observed the biological characteristics in ZJJH, compared the morphological characteristics of female adults of the three geological populations, investigated the genetic diversity and genetic variation of the three geographic populations by using RAPD-PCR technology with4random primers.The results showed that M. matsumurae belongs to sexual dimorphism and it happens two generations per year; the morphological characteristics of female adults in ZJJH and SDQD are more similar, especially the length of crested claws are resembled; the results indicted that DNA extracted from single individual of female adult was enough for RAPD-PCR, the genome size of the species was9416bp; in species level, coefficient of genetic differentiation (Gst) of the three repeats was0.2078,0.1919and0.2075, respectively, which indicates that20.78%,19.19%and20.75%of genetic diversity resided among populations, and79.22%,80.81%and79.25%of genetic diversity were within populations; the minimum genetic distance of the three repeats are0.0407,0.0355and0.0449between ZJJH and SDQD, respectively, and the maximum ones are0.0650,0.0562and0.0673between SDQD and LNFS, which suggested that the three geographic populations are very close in genetic relationship and that they are the same species; according to the UPGMA dendrograms, the3populations could be grouped into two clades and one contained ZJJH and SDQD and the other contained LNFS only, which indicated a closer genetic relationship between the populations of ZJJH and SDQD.This research first provides a scientific proof from morphology and genetic molecule to make population differentiation and species identification clear. It supplys scientific basis for the pest management. It is significant for the biological control in the future by using sex pheromones and the natural enemies of pine bast scale.