| Soil salinization is one of the important environmental issues in the world. Salinity stress can seriously affect plant growth and development, production and distribution. Puccinellia tenuiflora as a kind of the strong salinity ability monocot salt-tolerant plants, is considered as an ideal model plant for studying the salt-tolerant mechanisms in plants. In this study, we combined morphological and proteomic strategies investigated the short-term Na2CO3stress responsive through the differentially expression of the number of protein expression and expression abundance, then analysis of the differentially expressed proteins in different pI and molecular weight range differentially distribution characteristics. We found that short-term Na2CO3stress can inhibit the growth of Puccinellia tenuiflora seedlings, the plants grow slowly, wilting and dry phenomenon, and the greater the stress, the more obvious inhibition. Proteomic analysis of156differentially expressed protein spots. Of these,34spots differentially expressed(18spots up-regulated and16spots down-regulated) when150mm-12h Na2CO3stress treatment group compared with control group.41spots differentially expressed(20spots up-regulated and21spots down-regulated) when200mm-12h Na2CO3stress treatment group compared with control group.52spots differentially expressed(27spots up-regulated and25spots down-regulated) when150mm-24h Na2CO3stress treatment group compared with control group.58spots differentially expressed(25spots up-regulated and33spots down-regulated) when200mm-24h Na2CO3stress treatment group compared with control group. In the short-term Na2CO3stress conditions Puccinellia tenuiflora to mobilize a variety of proteins involved in salt stress response. These proteins may be through the following mechanisms to respond to short-term Na2CO3stress.Include:reduce photosynthesis, excite a variety of antioxidant mechanisms, increase the supply of energy and other metabolic pathways. |
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