Distribution Of Four PRRSV Receptors In The Central Nervous System Of Piglet

In order to research the distribution and expression of porcine reproductive andrespiratory syndrome virus(PRRS) receptors in the central nervous system of piglets, double-labeled immunofluorescence and western blot method were used to detect the subcellulardistribution and the relative expression of differences of PRRS virus respectively. The mainresults are as follows:1. PRRSV could cause widespread damages with a prominent feature of nonsuppurativeencephalitis, which was present with hyperemia, hemorrhage, degeneration, necrosis, glialcell proliferation in telencephalon, hippocampus, cerebellum and spinal cord.2. The results of immunochemistry SP method revealed that the PRRSV existed in thepiglets central nervous system and its target cells are neuron and astrocyte.3. The results of immunochemistry SP method and double immunofluorescencehistochemistry method revealed that all the protein of PRRSV receptors existed in the pigletscentral nervous system, especially in the neuron but only express a little or even does notexpress in glial cells. Sialoadhesin expressed in neuron of hippocampal and spinal cord butnot in other glial cells. Vimentin expressed in neurons and astrocytes but not in other glialcells. CD163expressed in neurons and oligodendrocyte but not in other glial cells. NMHCâ…¡-A expressed in neurons but not in glial cells.4. In various organs of piglets central nervous system, the expression of PRRSVreceptors were different from each other. Western Blot results showed that the receptorexpression levels increased slightly after virus infection and it might be the reason of PRRSvirus stimulus and subsequently persistent infection.To sum up, porcine reproductive and respiratory syndrome virus receptors expressed inpiglets central nervous system, and the expression had a process of dynamic change. Aftervirus infection, receptors expression quantity increases and had a certain influence for viruspersistent infection. It provided a new theoretical basis for the reveal of porcine reproductiveand respiratory syndrome virus infection, maintaining and blocking mechanism, also laid afoundation for researching and developing a new type of PRRSV receptor blockers.