QTL Analysis On Agronomic Traits Using Recombinant Inbred Line Population Of Hekezijing/Suyunuo

Many of the important agronomic traits in rice, such as plant height, panicle shape, grain shape, and etc, are quantitative characters controlled by multi-genes, showing continuous variations in segregating generations, and influenced greatly by environment and genetic background. Constructing molecular marker linkage map and development of genetic statisical model facilitate research of QTL mapping and cloning.In this study, QTLs for10agronomic traits were analyzed, based on the molecular linkage map and phenotype of Heikezijing/Suyunuo RIL population composed of162lines. The results are as follows:Three QTLs for grain length, all originated from the large grain cultivar Suyunuo, were detected. qGL1was located at interval of RM1254-RM3652on chromosome1, explaining5.5%of phenotypic variance; A major QTL, qGL3, explaining29.2%of phenotypic variance, was located between SSR marker of RM6959-RM6832on chromosome3, where is consistent with the positon of the cloned gene for grain length GS3; Another major QTL, qGL10, was located at interval of RM2125-RM8207of chromosome10, explaining30.9%of phenotypic variance with a LOD value of13.14, which was a newly discovered QTL for grain length. Besides, QTL qGW8for grain width was located at interval of RM5353-RM376, explaining7.8%of phenotypic variance.Three QTL regulating the glume color from Heikezijing was detected on chromosome4. A new QTL, qGC14.1, was located at interval of RM16616-RM16973, explaining67.9%of phenotypic variance with LOD value of11.47; qGCl4.2was located between SSR RM16973-RM3367interval, explaining68.9%of phenotypic variance with LOD value of13.24, where was in the range of a cloned glume color gene Bh4; qGCl4,3was located at RM241-RM6089interval, explaining17.7%of phenotypic variance with LOD value of4.54.A major QTL qSCl7controlling the peel color from Heikezijing was detected at RM180-RM5481interval, explaining52.4%of phenotypic variance with LOD value of12.79. this loci is consistent with the site of cloned gene RC for pericarp color.A QTL for awn length, qAL11, was detected at interval of RM3701-RM3428, explaining7.6% of phenotypic variance with LOD value of2.53.qEL3, a QTL for panicle length from Suyunuo, was detected at RM3513-RM8277interval on chromosome3, explaining16.9%of phenotypic variance with LOD value of2.79. Also, qPE7, a QTL affecting spike exsertion, was detected between SSR marker of RM336and RM351on chromosome7, explaining8.2%of phenotypic variance with LOD value of3.02.qPH6, a QTL regulating plant height, was detected between RM7561-RM190at the terminal of long arm of chromosome6. It explained12.07%of phenotypic variance with LOD valueof2.63and additive effect of-6.962, indicating that increasing effect came from Suyunuo. qFW6, a QTL for leaf width was detected between RM3567-RM454on chromosome6, with the LOD value of3.12and explaining9.62%of phenotypic variance.