Isolation, Purification Of S-Adenosyl-L-Methionine And Preparation Of Sam Sulphate

S-adenosyl-L-methionone(SAM, also known as Ado-Met, SAMe), an important metabolic intermediate, is widely found in various organisms. The cell disruption extraction methods of SAM and the separation and purification of SAM were studied in this paper, together with the preparation of SAM sulphate and it’s stability.The study on the cell disruption extraction methods of SAM indicated, formic acid extraction was the best for extraction, and the optimal conditions was:concentration of formic acid:0.5mo1/L, the amount of formic acid:1.25BV, broken time:90min. The SAM extraction rate can reach more than95%.Compared with the traditional way of using picric acid or picrolonic acid to precipitate SAM selectively, this method not only increased the recovery, but also diminished the operation risk and the environmental pollution. It was shown in the study that the optimal pH condition of SAM in the D113weakly acidic cation exchange resin was pH5.0. Using sulphuric acid to elute SAM and suitable elution condition was found as follows:sulphuric acid concentration:0.2mo1/L, flow rate:2BV/h. The recovery of SAM reached90.3%.The reversal phase precipitation method was used in the preparation of SAM sulphate. It was shown in the study that the suitable crystallization condition was found as follows:the methanol ratio:80%, the amount of methanol:5BV. The yield of SAM sulphate reached more than70%with the purity of91.05%.The stability of SAM sulphate was still affected by the pH and temperature, but the temperature has great influence on the stability of SAM sulphate. With increasing temperature, the degradation velocity was significantly accelerated and the loss of SAM increased. So, the SAM sulphate should be stored at low temperature(under0℃).This paper aimed at improving the SAM isolation and purification method, the extraction rate of SAM, optimizing the whole process, reducing the loss of SAM, establishing a good foundation for industrial application.