Study On Preparing Of Zinc Chelating Fish Scale Small Peptides

Fish scales contains abundant of collagen protein, which is a kind of biologicalmacromolecule protein, and it is necessary nutrition to help people to resistanceanti-aging. When protein is decomposed into small peptides, it can be absorbed bybody, when the molecular mass of small peptides is under1000Da, the small peptidescan be directly absorbed by human body without decomposition. Zinc chelating smallpeptides can be stable in small intestine where is alkaline, not only it can complementproteins, but also it can complement zinc.China’s aquatic products processing industries growing maturity stage by stage.In the processing of aquatic products, it produced a lot of rich in nutrition byproductlike fish scales of the present, but these byproduct have not be reasonably andeffectively used, it not only causes the waste of resources, but also leads to thepollution of environment. So byproduct of aquatic products being made full use of isan important and far-reaching significance for aquatic products processing industry.This literary studied the production of fish scales being used to prepare collagen smallpeptides and zinc chelating small peptides.The main influence fo extracting collagenfrom fish scale, enzymolysis technology of collagen peptide and the chelating of zincand small peptides was certainly explored and studied, the physical study and analysisof product was made. The main results are as follows:(1) Hot water was used to extract collagen from fish scale, the extractiontemperature, extraction time and ratio of material to solvent were main influence ofextracting, When extraction temperature of solution was70℃, the time of extractionwas6hours and ratio of material to solvent was1:20, the optimum extraction processwas obtained, and the maximum yield was32.55%.(2) Alkaline protease was used to enzymolysis of collagen protein, the time, thetemperature,the pH of solution and amount of enzyme were main influence ofenzymolysis technology. When the time of reaction was1.5hours, the temperature ofsolution was55℃, the pH of solution was9.5and amount of enzyme was0.3%(v/v),the optimum extraction process of collagen enzyme was obtained, and the largestdegree of hydrolysis was23.17%.(3) Automatic amino acid analyzer was used to measure amino acids of collagenpolypeptide distribution, its amino acid composition was in accord with thecharacteristic of collagen. Gel permeation chromatography was used to determine themolecular mass of collagen peptide, most of the molecular mass of collagen peptidewas within1000Da.(4) The aqueous-phase way was used to chelating reaction of zinc and smallpeptides, the time, temperature, the pH of solution and mole ratios of small peptidesto zinc were main influence. When the time of reaction was75min, the temperatureof solution was70℃, the pH of solution was6.5and mole ratios of small peptides tozinc was2:1.2, in the chelate product, the weight ratios of zinc to small peptides was 5.79%.(5) IR was used to analysis substance molecular structure of chelating reactionbefore and after, it was found that the carboxyl and amino was changed. The UVscanning was used to the small peptides of chelating reaction before and after, theabsorption of small peptides was changed, it showed the zinc small peptides hadreacted. The molecular mass of small peptides smaller, the chelating reaction waseasier, the solubility and stability of zinc chelating small peptides was well.