Studies On Xanthone Derivatives Of Endophytic Fungus Ynca0351from Parisl

Paris belong to Phomopsis genus, was a perennial herb which prevalent inYunnan, Szechwan, Kweichow. Paris was a rare medicinal plant. Endophyticfungus Paris attacks Paris, these microorganisms produce a number of secondarymetabolites with various biological activities, including antimicrobial, antifungal,antimalarial, antitumor, and the like. The xanthone derivatives are importantmetabolites isolated from the Phomopsis genus, and they appeals to medicinalchemists because of their pronounced pharmacological effects. With the aim ofmultipurpose utilization endophytic fungus of isolated from the rhizome of Paris var.yunnanensis and identify bioactive natural products, the phytochemical investigationon fermentation products of the endophytic fungus Phomopsis amygdali.was carriedout. As a result, fifteen new dihydroxanthones (1*-15*), together with twenty fourknown dihydroxanthones (16-39), were isolated from this extract. The structures ofnew compounds were elucidated on the basis of a comprehensive analysis of the1HNMR,13C NMR and2D NMR spectra, results are as follows:methyl-2-hydroxy-6-(hydroxymethyl)-8-methoxy-9-oxo-2,9-dihydro-1H-xanthene-1-carboxylate (1*), methyl2-hydroxy-8-methoxy-6-methyl-9-oxo-2,9-dihydro-1H-xanthene-1-carboxylate (2*), methyl-2,8-dihydroxy-6-(2-hydroxyethyl)-9-oxo-2,9-dihydro-1H-xanthene-1-carboxylate (3*),4,5-Dihydroxy-3-(2-hydroxyethyl)-1-methoxy-5-methoxycarbonylxanthone (4*),1,8-Dihydroxy-4-(2-hydroxyethyl)-3-methoxyxanthone (5*),1,5-Dydroxy-3-ethanol-6-methoxycarbonyl-xanthone (6*),1-Hdroxy-5-methoxy-3-et-hanol-6-methoxycarbonyl-xanthone (7*),1-Hydroxy-3-ethanol-8-ethoxycarbonyl-xanthone (8*),1,5-Dihydroxy-3-(2-oxopropyl)-6-methoxycarbonyl-xanthone (9*),1-Hydroxy-3-(2-oxopropyl)-8-methoxycarbonyl-xanthoe (10*),1,4,8-TrI hydroxy-3-methoxy-5-(1,3,4-trihydroxybutan-2-yl)-xanthone (11*),1,3,4-Trihydroxy-8-methoxy-5-(1,3,4-trihydroxybutan-2-yl)-xanthone (12*),6-hydroxy-1-methoxy-8-methoxycarbonyl-3-(1,3,4-trihydroxybutan-2-yl)-xanthone (13*),1-hydroxy-5,6-twomethoxy-8-methoxycarbonyl-3-(1,3,4-trihydroxybutan-2-yl)-xanthone (14*), paucinervin G (15*), AGI-B4(16), globosuxanthone A (17), nidulalin A (18), pinselin(19),1-hydroxy-4,7-dimethoxy-6-(3-oxobutyl)xanthone (20),1,5-dihydroxy-3-(2-Oxopropynl)-6-methoxycarbonylxanthone (21), paucinervin E (22), asperxanthone (23),6-O-methyl-2-deprenylrheedIa-xanthone B (24),1-hydroxy-8-(hydroxymethyl)-3-methoxy-6-methylxanthone (25),1,7-dihydroxy-2-methoxy-3-(3-methylbut-2-enyl)xanthone (26),1-hydroxy-4,7-dImethoxy-6-(3-oxobutyl)xanthone (27), Asperxanthone(28), secosterigmatocystin (29),6-O-methyl-2-deprenylrheediaxanthone B (30),1,8-dihydrox y-4-(2-hydroxyethy)-3-methoxyxanthone (31), pinselin (32), icathin B(33), secosterigmatocystin (34), garcInexanthone F (35), hypericumxanthone A (36),dihydrosterigmatocystin (37), secosterigmatocystin (38), vieillardixanthone (39). Inaddition, cytotoxicities of compounds1*-15*were evaluated. The compounds1*-15*were tested their cytotoxicity against NB4, A549, SHSY5Y, PC3, and MCF7tumor cell lines.