Investigation Of Self-assembly Film Of Chitosan Molecule And Composite Material With Atomic Force Microscopy

Chitosan is a natural polymer from renewable resources, obtained from shell of shellfish, and thewastes of the seafood industry. Chitosan has been found a fascinating candidate in a broad spectrum ofapplications along with unique biological properties including biocompatibility, biodegradability toharmless products, nontoxicity, physiological inertness, remarkable affinity to proteins, antibacterial,haemostatic, fungistatic and antitumoral properties. Molecule self-assembly technology has been widelyapplied in biotechnology and nanotechnology. As a fabrication tool, molecular self-assembly technologywill become tremendously important in the coming decades. In this article, mechanism, influence factors,some research advances and application of chitosan film molecule self-assembly technology are studied.Chitosan is a natural polymer with biocompatibility and biodegradability. Recognition of the potentialutility of chitosan self-assembly film is growing and the field of chitosan biomaterials is poised toexperience rapid growth. Recent studies suggested that chitosan self-assembly film is promising candidatesas a supporting material for tissue engineering applications owing to their porous structure, gel formingproperties, ease of chemical modification, high affinity to in vivo macromolecules, and so on. In this review,people focus on the various types of chitosan self-assembly film and their use in various tissue engineeringapplications namely, skin, bone, cartilage, liver, nerve and blood vessel. The field of wound healing hasbeen a major emphasis in chitosan-based medical applications research. Chitosan self-assembly filmpossesses the properties favorable for promoting rapid dermal regeneration and accelerate wound healingsuitable for applications extending from simple wound coverings to sophisticated artificial skin matrices.In this paper, first we use new methods to preparation of chitosan monolayer film and by using theAFM characterization developed it. Secondly，three kinds of chitosan with different molecular weight werechosen to systematically investigate their self-assembly behavior. Through high resolution atomic forcemicroscopy (AFM) technique, we have characterized the self-assembled morphologies of three acidulatedchitosan molecules at designed culture time. The AFM characterization results suggest that the molecularweight has direct influence on the self-assembled pathway of chitosan molecules, therefore leading toformation of chitosan films with different properties. At last, we use different methods of preparation of chitosan composite film. And we explored the properties. This paper analyses the properties of chitosanself-assembly film which are important to biomaterials.In our experiments, the contents are listed as follows:1. Using AFM scanning technology by adjusting the concentration of chitosan solution and theincubation time, preparation of ultrathin monolayer chitosan biological membrane.2. The preparation chitosan film by molecular self-assembly method. Influence of molecular weighton the self-assembly film of chitosan studied by atomic force microscope.3. The preparation of composite films with chitosan, and the exploration of the mechanism.