Study On The Distribution Pattern Of Archaea And Methanotrophs Community In Tianshan No.1Glacier Peripheral Permafrost Active Iayer

Object：The environment of the sediments in the bottom of the glacier is relatively isolated,there is distincttime sequence succession in it’s degenerate frontier environment, this geographical habitats providesexcellent natural conditions for researching and understanding the biological succession process，geographic patternsthe， ecological effects of the functional groups and their area response of globalchange of the original ecosystem which less vulnerable to the impact of human activities.Clarify themicroorganisms in the glacier environment not only contribute to research and development oflow-temperature microbial technology, but also provide the basic data for understanding thebiogeochemical processes occurring in the bottom of the glacier deposited layer of low-temperatureenvironment.Methods：In this study, using PCR technology to clone the methane bacteria pmoA gene, as well as usinghigh-throughput sequencing and denaturing gradient gel electrophoresis (DGGE) technology to analysisArchaea in the forefront environmental of glacier and archaeal community structure in alpine meadowspermafrost active layer. By PCR amplified, sequencing, sequence analysis, phylogenetic tree to determinethe structure and composition of the microbial communities in different soil samples.Results：Our main results are as follows：1. There are total of51OTU in the Glacier No.1in the Tianshan Mountains. Results of phylogenetic showthe vast majority methane oxidizing bacteria that detection sequence represented are still uncultured taxa inthe alpine meadow permafrost active layer of Tianshan Glacier forefront the,which including Upland SoilCluster α (USCα), Upland Soil Cluster γ (USCγ), Cluster1(CL1) into three categories. And Upland, Soilthe Cluster gamma (USCγ) Cluster1(CL1) is the dominant group.Part of the Upland Soil Cluster γ (USCγ)OTU accounted for more than50%.While Upland Soil Cluster α (USCα) taxa detected only4OTU.2. There are certain abundance of archaea in the environment forefront of the glacier, but overall lowabundance.We analysis the community structurer of achaeal in the forefront environment of the TianshanNo.1Glacier and found that, with the time series of cutting-edge environment, archaeal diversity overallincrease. We detected the Halobacteriaceae,Methanosarcinaceae and Methanobacteriaceae whichsemidetached part Euryarchaeota.The sequence abundances ratio of the Halobacteriaceae is significantlyhigher in early and interim soil than later of mature soil. This may be because early soil is mainly baremoraines, inorganic salt component is greater, to provide more favorable conditions for the survival ofhalobacteria. There is already evidence that the group1.1b and group1.1c taxa widesprea，they aresemidetached part nonthermophilic Crenarchacola play an important function in the nitrification of soilnitrogen. However, the role of biogeochemical cycles of archaea lack of system reports in the environmentforefront of the glacier. Currently number of cultures of detachable archaea so few that phylogeneticattribution of considerable part of the sequencing sequence can not be clearly identified. To really understand the community structure and function of archaeal in the environment forefront of the glacierremains to be in-depth study.3. Although Euryarchaeota was more abundant in both the young and intermediate soils, Crenarchaeotashowed a dominance of over the whole Glacier No.1forefield.Conclusion：Results of phylogenetic show the vast majority methane oxidizing bacteria that detectionsequence represented are still uncultured taxa in the alpine meadow permafrost active layer of TianshanGlacier forefront the. Low numbers and diversity of both Euryarchaeota and Crenarchaeota were found insediments beneath the Glacier No.1in the Tianshan Mountains. Our analysis revealed a numble of readscould not be assigned to specific taxa of archaeota. Although Euryarchaeota was more abundant in both theyoung and intermediate soils, Crenarchaeota showed a dominance of over the whole Glacier No.1forefield.At the family level, reads assigned to Euryarchaeota were affiliated with family Halobacteriaceae、Methanosarcinaceae、Methanobacteriaceae.