Electrochemical Immunosenor Based On Functional Nanomaterials

In recent years, the rapid development of nanotechnology, makes thedevelopment of subjects, emerging research areas and hot spots onnanomaterials. The combination of nanotechnology and analyticalchemistry is a good opportunity to Modern analytical chemistry. Theemergence of nanotechnology has opened a new direction for thedevelopment of electrochemical biosensor. How excellent physical andchemical properties of functional nanomaterials to be used in theelectrochemical sensor is a research focus.This paper studies around on the application of Functionalnanomaterials in electrochemical immunosensor and the main contentsare as follows:The1chapter, Introduction, We briefly introduce concept ofclassifications of the immunosensor, the main immobilization methods ofbiological molecules and signaling molecules, and functions ofnanomaterials in sensors.The2chapter, Au nanoparticles(AuNP)-Polydopamine(PDA) as thesensor platform, multienzyme-antibody functionalized AuNP-PDA@carbon nanotubes (CNT) as the signal labeling, wrere applied tothe immunesensing study of Interleukin-6.The3chapter, We prepared gold nanoparticles-polydopaminemagnetic nanoparticles composite nanomaterials and characterized it.Stripping of gold was detected Interleukin-6. Hemoglobin was employedto block active sites against nonspecific binding and direct electrontransfer. The4chapter, We prepared gold nanoparticles-polydopamine@carbon microsphere characterized it. For Hg2+can make Thyminemismatch, we design specific DNA sequence to make the sensor candetected Hg2+and achieved electrical contacted enzyme.The5chapter, we designed sandwich DNA-multiple enzymesstructure as label of sandwich type immunosensor, signal was amplifiercompared with the traditional sandwich immunosensor.The6chapter, we successfully perpared AuNP/L-cysteine/graphenenanocomposite materials, and successfully applied to the detection ofHIgG.