Eye Needle Treatment Of Cerebral Ischemia Reperfusion Injury In Rats Of Influencing The Expression Of Bfgf, Mmp - 9 Experimental Observation

Purpose：The needle treatment eye cerebral ischemia and reperfusion injurycauses of pathological mechanism， and to observe the eye alkaline fibroblastgrowth factor（bFGF），matrix metalloproteinases（MMP-9）influence，accordingto the test results the try to find effective method reduces cerebralischemia-reperfusion injury， treatment of cerebral ischemia disease hasclinical significance.MMP-9C1562TT alleles are thought to cerebral ischemia and incidence ofintracerebral hemorrhage about， when cerebral ischemia and cerebral hemorrhagein onset increased T alleles， it will lead to gene transcription enhancement，protein expression increased， thus MMP-9in cerebral ischemia and whenintracerebral hemorrhage increased significantly， the increased serum level，cause brain the increase of blood capillary permeability， can cause thedestruction of the blood brain barrier and the permeability， leading to lossmyelin， cause brain damage. When ischemic brain damage occurred in the sametime， the change will start endogenous protection mechanism of stress，induction neurotrophic factor protection repair damaged nerve. BFGF is one ofneurotrophic factors， can fight the neurons apoptosis， inhibition ofexcitability of amino acids toxicity and stabilizes intracellular Ca2=balancedand enhance the hippocampus respond to ischemia resistant， and bFGF also enhancecultured hippocanlpal neurons excitability of toxic amino acids tolerated.Eye of the needle，is our national old doctor of traditional Chinese medicineJingShan peng professor of traditional Chinese medicine theory basis foundedthe meridian out.Clinical applications for more than30years to， the treatment of strokecurative effect is distinct， obviously decrease the stroke patients of morbidity， improve the living quantity， reduce the burden on society. Thisexperiment through the observation MMP a9and bFGF in rats ischemia andreperfusion tissue of expression， aim at for eye of the needle for popularizingscientific research to provide the scientific basis for the experiment.Objects and methods：Will SD rats40only according to random principleaverage into four groups and were blank control， model group， eye needle group，TiZhen groups， and the only10. In line with the brain artery bolt for legalsystem focal cerebral ischemia and reperfusion in the rat model. Keep the ratfocal cerebral ischemia after2h reperfusion， in24h reperfusion aftertreatment for observation， the assessment standards Longa， eligible into thenext operation， attention for not qualified or at any time added death out ofrats， ensure that each group of10only. Blank control group： health SD ratsnot to processing， normal feed， don’t give any treatment measures. Model group：build model success， the normal feed， don’t give any treatment measures. Eyeof the needle： made after the success of the mould fabrication，24h afterpouring in to needle treatment one eye. Select28，0.5"HaoZhen， acupunctureeye OuDeShuang side， middle area， in the liver and kidney area acupuncturepoint， middle area and liver area for discharge method using flat flat， LiuZhen25min， LiuZhen period no needle. TiZhen way： made after the success of themould fabrication， reperfusion after24hours to TiZhen treatment time， takethe gutters， foot three mile. To meet the requirements of the rats in quicklytake brain， and then used ELISA method （ELISA） test MMP-9and bFGF positiveexpression levelResult：The results show that from using ELISA method （ELISA） ultra-thinslices of the brain with the observation MMP-9and bFGF positive expressionlevel： MMP-9in model group express the most， the eye of the needle and TiZhengroup decreased to the eye of the needle express cut the most obvious. In themodel group in bFGF expression is not clear in the eyes TiZhen needle， the treatment group bFGF expression are enhanced， among them with eye needletreatment group raised the most obvious.Conclusion：Eye needle treatment of cerebral ischemia and reperfusion tissuedamage when the mechanism is by inhibiting MMP-9of expression， to reduce theblood brain barrier damage， restrain damage of apoptosis neurons； Through theincrease of bFGF expression， to speed up the ischemia repair injured tissueand regeneration of new blood vessels， and on the cerebral ischemia andreperfusion tissue of protection.