The New Body Pain By Stasis Soup Experimental Research For The Treatment Of Acute Spinal Cord Injury

Objective: to observe and analyze the new body pain to rat out silting soupafter acute spinal cord injury hypoxia-inducible factor influence of a1a,explore the active body pain by silt decoction "in the treatment of acute spinalcord injury to promote rats functional recovery of function and molecularmechanism for the new body pain, sedimentation by decoction to spinal cord injuryto provide theoretical and experimental basis.Materials and methods:60SD rats (China Medical University ExperimentalAnimal Center), weight125persons20g, male or female, were randomly dividedinto control group, hormone therapy group and treatment group group (injury ld,3d,5d,14d). Experimental group to establish rat HIP spinal cord injury model,injury in rats T10spinal cord, the rats in each group to the corresponding pointin time were killed, the use of40stay L paraformaldehyde perfusion fixed andsegments the removal of the damage spinal cord approximately15.0mm HE stainingand immunohistochemical staining methods. Detection of secondary spinal cordinjury, spinal cord tissues of the pathological changes of circumstances anddifferent time after injury, HIF-1a timing of expression. Results found: brownparticles were positive in the cytoplasm or nucleus. Each slice optional10vision, to count the number of positive cells. Data using SPSSVn packagetreatment.Chinese medicine treatment groups:(new body pain Zhu Yu Tang, LiaoningUniversity of Traditional Chinese Medicine)6ml/kg/d, intraperitonealinjection;Hormone treatment groups:(methylprednisolone sodium succinateinjection, the Belgian strong company production)30mg/kg/d, intraperitonealinjection;Control group:(saline)6ml/kg/d, intraperitoneal injection of Static spinal cord injury model: high-pressure disinfection equipment packageafter3hours,3%pentobarbital sodium abdominal anesthesia SD rats (drugconcentration30ml/kg), anesthetic success later, the rats prone fixed on theanimal fixed board. Surgery under a microscope, No.10thoracic center ofshearing, the range of about5*3cm, iodine alcohol skin disinfection, surgicaltowels, shop, the back of the line longitudinal midline incision of about4cm,blunt dissection of subcutaneous and muscle, hemostasis, exposure10thoracicupper and lower lamina, a small rongeur bite in addition to the lamina, and fullyexposed the spinal cord dorsal and sides, to maintain integrity of the dura.Weight of30g (special weights,30g of quality, access to an area of10.0mm*1.0mm)8minutes of local oppression, oppression, and epidural, the contactsurface was10.0mm*1.0mm, placed in the dorsal midline, the vertical pressure.Body spasmodic quiver when the oppression of the following conditions are met,the tail spastic swing, lower limbs and body retraction kind of flutter; seeintradural congestion or edema, compression of the lower limbs were flaccidparalysis, do the inclined plane test, determination of maximum angle <30°as the standard. Laminectomy. Suture muscle and skin. SCI animals after surgeryperformance paralysis of the lower limbs, bladder distention, a day to beartificial massage micturition defecation; all animals given the same diet andlight. Rats in each group were sacrificed after a predetermined period ofobservation, the paraformaldehyde perfusion-fixed, take the damage spinal cordtissue is approximately10.0mm, respectively, at-80℃to save. Tissue fixationand slicing:1.4HE staining and immunohistochemical staining. Statisticalanalysis Data were expressed as mean±standard deviation, the applicationSPSSvll.5statistical software variance analysis by HE staining,immunohistochemistry and electron microscopy to observe the expression of HIF-1ain the detection of spinal cord tissue factor.Results: three days later HE staining showed that the pathological changesof the3days after the new body pain Decoction group of spinal cord injury islighter than the saline group, compared with methylprednisolone re-injectiongroup, immunohistochemistry showed that HIF-1a cells were mainly found in neurons in the cytoplasm and nucleuswithin, etc., mainly in the spinal anteriorhorn neurons and a small number of glial cells in the expression of damage localneurons, the cytoplasm deep eosin staining, nuclear condensation, cell bodyshrink. Damage control rats after spinal cord injury, these cells expressincreased,2d-3d and reached the peak level and then gradually decreased tonormal levels; body pain Decoction group, the spinal cord of rats with HIF-1aexpression than in group B is low, the peak also occurred2d-3d body pain afterinjury Decoction group and saline injection group between the two groups after12hours there is no difference, the control group and treatment difference p<0.05after24hours,72hours later, two groups have significant difference(p <0.01). HIF-1a expression in the rat spinal cord tissue; new body painDecoction group no significant differences in the control group of12hours withB-type injury, reached a peak level24h after injury, but its expression levelis weak than that of group B, and gradually diminished with time.Conclusions: acute traumatic spinal cord injury, the new body sedimentation soupcan inhibit pain by hypoxia-inducible activity expression, a1a after acutespinal cord injury to restrain the secondary damage, reduce a1a mediatedhypoxia-inducible has beneficial effects on the inflammatory response to spinalcord injury has certain protective effect.