Vitiligo Light Aging Degree Affect The Relevance Of Research

Objective The research is aimed to investigate the diffrences between vitiligo and the normal skin, by measuring laser induced fluorescence spectrum. Find new methods to auxiliary diagnosis vitiligo. And investigate the signification of the vitiligo character fluorescence spectrum.Materials and methods Firstly patients are definite diagnosed as vitiligo. Identify the involved area. Then with a365nm light emitting diode (LED), measure the laser reflectivity of vitiligo, using Ocean Optic USB2+F02243spectrometer. The reflectivity of365nm is measured. And normal skin is measured in the same way. Find diffrences of the two areas in one patient. To other patients, The Laser Induced Fluorescence Spectrum of vitiligo is measured, using Ocean Optic USB2+F02243spectrometer. The fluorescence spectrum extent is between350nm and1000nm. And measure normal skin in the same way. Find diffrences of two areas in one patient.Results Reflectivity of vitiligo is higher than normal skin. Vitiligo reflectivity to normal skin reflectivity is11%-23%. The spectrograph of vitiligo has two fluorescence peaks at358nm and491nm. And the fluorescence intensity of vitiligo is higher between350nm and1000nm compared to normal skin. The P value of spectral intensity between vitiligo and normal skin is zero. And it is normal distribution. Maximal value is855.1282%, mean is99.9773.Conclusions Vitiligo lesions are detected two fluorescence peaks at358nm and491nm. Conduce to assistant diagnose of vitiligo. Melanin and melanocyte absorb light radiation, and may relieve or prevent photochemical reaction, play a part in relieving UV induced skin damage. Objective To investigate the process of skin photoaging is promoted or postponed because the melanocyte and melanin are damaged, when vitiligo involved in the face.Materials and methods firstly with skin analysis System pictures of patients are taken to diagnose vitiligo definitely. Then guide patient take pictures of the lesion and the symmetrical area. Local imagines are magnified5to10times separately, including lesions and the normal symmetrical areas. The quantity and depth of wrinkle are evaluated, which demonstrate the degree of skin photoaging. Evaluate the severity of skin photoaging between vitiligo lesions and normal skin.Results192patients with vitiligo are investigated. And259paired areas are compared.59photoaging of vitiligo are more severe than normal areas, taken22.8%;128have no diffrences, taken49.4%;72photoaging of vitiligo are slighter than normal areas, taken27.8%. To patients older than40years, we do corrected χ2analysis. χ2value is0.13, and0.5<P<0.75. There are no ststistical significance of skin wrinkle in people older than40years with vitiligo.Conclusions Because the course of vitiligo is short, about half of patients have no diffrences between vitiligo lesions and normal skin. The ratio of vitiligo with more severe skin photoaging is slightly lower than vitiligo with slighter photoaging. The author concludes vitiligo may postpone the process of skin photoaging, but still need deeper investigation. Objective The research is to set up vitiligo model using external hydrogen peroxide (H2O2) application.Materials and methods Firstly C57BL/6black mice are chosen as experimental model. The hair of mouse’s back is shaved. The bare area is divided to four rigions. One region use external5%H2O2application to local blanch, twice a day. Another region use external10%H2O2application in the same way. The rest rigions treat without any intervention. Shave hair regulary, experimental session keeps at least two months. Four mice were undertaken the experiment.Results During the experiment there are crusts forming in the intervent areas. After thick crust exfoliated may form scar. At the end of experiment no hypopigmentation or leukoplakia was found in5%H2O2rigions, and without hypopigmentation or leukoplakia in10%H2O2rigions. With ultraviolet lamp there is no bright white fluorescence. Histopathology revealed epiderm thicking, keratinocytes contain pigment granules. No obviouse hypopigmentation in epiderm layer. Dermis collagen layer is thicking. The arrangement of collagen fiber is compact and disordered. There are pigmentophage in the dermis. S-100stainning manifeste the quality of melanocytes decreased slightly.Conclusions H2O2induce the melanocyte damaged. And also induce epidermal thicking and remoding of connective tissues, which is not consistant in vitiligo. By using external H2O2application for two months we can not set up vitiligo model. Objective C57BL/6mice and BALB/c mice are received simulative solar irradiation. Then investigate the associativity between skin melanosome and the degree of skin photoaging.Materials and methods Type SUV-1000solar ultraviolet simulator emission simulative solar is used. C57BL/6mice and BALB/c mice are accepted same irradiation for12weeks. Light intensity is suberythema dose. The control group is without any treatment. Observe the coarse wrinkle of the skin, and take histology examination at the end of experiment, observing the degree of photoaging.Results C57BL/6mice are with colour skin, the melanosome content are more than BALB/c mice. After irradiation the skin become coarse, dry and covered with fine scales. Pathology revealed corneum is thicking and compact. Epidermal is composed with5-10layers of keratinocytes. Keratinocytes with vacuity is increased in number. The skin goffers become flat. The arrangement of collagen is compact, with unclear definition. The collagen interspace disappeared. Eosinphile mass is deposote. And in the dermis there are scaterred histiocytes. Masson stain revealed light blue collagen fibers. Collagen bundle become thinner and fragment. The border is not clear, the polarity disappeared. The interspaces between collagen bundles nearly disappeared. Deposition is slightely increased around elastic fibers. The intervention group’s differnces between C57BL/6and BALB/c are not obviouse with HE stainning.Conclusions The photoaging model can be found in12weeks. The degrees of photoaging in mice skin coloure difference can not be found in12weeks with HE and Masson stain. A longer time experiment (with/or weaker light intensity) maybe make sense. The evaluation of skin photoaging degree is difficult at present, which need further investigation.