Splenectomy For Liver Fibrosis Rats Serum Timp 1 And Timp - 1 Level

Objectives:To discuss the effects of splenectomy on Hepatic fibrosis. Try to study the effects of splenenctomy on the expression of TIMP-1in the liver and the serum level of TIMP-1of the rats with hepatic fibrosis.Methods:By hypodermic injection CCl4,we established92rat model-s with hepatic fibrosis,and stopped injecting CC14from8th week.Splenec-tomy were performed in three groups at different phases.The control gro-ups were established at the same time.Then took samples of the livers and serum of the rats in different phases.The expressions of TIMP-1in the li-ver were detected by immunohistochemistry technique and the degree of hepatic fibrosis were detected by HE staining.The serum levels of TIMP-1were analyzed by ELISA technique.Results:1.TIMP-1positive material that lay in hepatic stellate cell,around the blood vessels and in the fibrous tissue were brown particles and were mainly distributed in the intracytoplasm of parenchymal cells.There is a little positive particle in fibrous tissue and minimal in normal tissue.We could detect the effects of splenectomy on hepatic fibrosis after HE staining:the progression of hepatic fibrosis in the first group is slow;the progression of hepatic fibrosis is also slow in the second group;The inflammation and fibrosis is relieved significantly in the third group. 2.the expression of TIMP-1in liver with splenectomy were less than that of the rats without splenectomy, and there were significant differenc-es; with the progress of liver fibrosis the expression of TIMP-1in liver g-radually increased (α=0.05). When the damage was stopped in liver cir-rhosis, the expression of TIMP-1in liver was not changed obviously.3. Serum TIMP-1in early and late liver fibrosis after splenectomy was significantly higher than control group;serum TIMP-1in midterm of liver fibrosis after splenectomy were significantly higher than the control group;with the progress of liver fibrosis, serum TIMP-1increased slowly (α=0.05).When the damage was stopped in liver cirrhosis,serum TIMP-1was decreased apparently.4.The trend of TIMP-1expressions change in their liver tissue and t-he serum TIMP-1levels,induced by splenectomy, are inconsistent, some-times even diametrically conflicting.The TIMP-1expression in rat liver a-fter splenectomy was significantly lower than the control group;in the ea-rly and late stage of liver fibrosis,serum TIMP-1level in rats underwent splenectomy after splenectomy increased more significantly than the con-trol group while serum TIMP1levels in midterm of liver fibrosis decreas-ed more significantly after splenectomy than the control group.Conclusion:1. Splenectomy can relieve hepatic fibrosis,and played an important role in the end-state of hepatic fibrosis. 2.With the development of hepatic fibrosis,the expressions of TIM-P-1in the spleen and serum of TIMP-1of the rats increased, when the damage is stopped in liver cirrhosis,the expression of TIMP-1in liver was increased a little and serum of TIMP-1was decreased apparently.3. the spleen influences liver fibrosis bidirectionally and differently on different stage of hepatic fibrosis.