Different Intensity Of Exercise Training On The Research On The Effects Of Rat Skeletal Muscle Energy Metabolism

1research purposesBy observing the different exercise intensity treadmill training in growing rats quadriceps ofSDH, MDH, LDH, and Ca²⁺-Mg²⁺-ATPase activity and muscle glycogen content to exploredifferent intensity treadmill training to improve energy metabolism andthe mechanism of actionof the athletic ability, for better guidance to athletes in training, and enhance athletic ability toprovide a scientific basis.2MethodsSelect the4-week-old male wistar rats70（Shandong Lukang Pharmaceutical Co., Ltd.）,average weight of67.99g±5.83g, according to the weight random match is divided into sevengroups （n=10）: the quiet control group and65%VO_2maxexercise group,70%VO_2maxexercisegroup,75%VO_2maxexercise group,80%of VO_2maxexercise group,85%VO_2maxexercise groupand90%VO_2maxexercise group.Treadmill Training for9weeks, were sacrificed to take thequadriceps determination related refers to indicators.All data SPSS17.0statistical software forstatistical analysis.3Results3.1experimental body weight of rats before and afterExperiment before the rats body weight was no significant difference （P>0.05）.70%VO_2maxexercise group after the experiment body weight was significantly lower than the control group（P <0.05）,75%VO_2maxexercise group,80%of VO_2maxexercise group,85%VO_2maxexercisegroup and90%VO_2maxexercise group is significantly lower than control group（P <0.01）,90%VO_2maxexercise group were significantly lower than the65%VO_2maxexercise group and70%VO_2maxexercise group （P <0.05）.3.2The quadriceps weight changes in rats65%VO_2maxexercise group,70%VO_2maxexercise group,75%V2maxresearch purposesBy observing the different exercise intensity treadmill training in growing rats quadriceps ofSDH, MDH, LDH, and Ca²⁺-Mg²⁺-ATPase activity and muscle glycogen content to exploredifferent intensity treadmill training to improve energy metabolism andthe mechanism of actionof the athletic ability, for better guidance to athletes in training, and enhance athletic ability toprovide a scientific basis.3.3each rat quadriceps muscle glycogen content changesNine weeks of treadmill training, muscle glycogen content of70%VO_2maxexercise groupvery significantly higher than that of65%VO_2maxexercise group,85%VO_2maxexercise group,90%of VO_2maxexercise group （P <0.01）,70%VO_2maxmovementgroup was significantly higher than the75%VO_2maxexercise group,80%VO_2maxexercise group two groups （P <0.05）.75%VO_2maxexercise group,80%VO_2maxexercise group difference was not significant.75%VO_2maxexercise group,80%VO_2maxexercise group was significantly higher than that of65%VO_2maxexercise group,85%VO_2maxexercise group,90%of VO_2maxexercise group （P <0.05）.3.4The changes in rat quadriceps lactate dehydrogenase activityLDH activity of70%VO_2maxexercise group,75%VO_2maxexercise group very significantlyhigher （P <0.01）,65%VO_2maxexercise group,80%of VO_2maxexercise group,85%VO_2maxexercise group,90%of VO_2maxexercise group significantlyhigher than that in the control group（P <0.05）;75%VO_2maxexercise group was significantly higher than the65%VO_2maxexercisegroup,80%VO_2maxexercise group,85%VO_2maxexercise group （P <0.05）;70%VO_2maxexercisegroup,75%between VO_2maxThe exercise group difference between the two groups was notsignificant.3.5groups of malate dehydrogenase activity in rat quadriceps changesMalate dehydrogenase （MDH） activity,70%VO_2maxexercise group,75%VO_2maxexercisegroup very significantly higher than that in the control group and90%VO_2maxexercise group （P<0.01）;65%VO_2maxexercise group,80%VO_2maxexercise group85%VO_2maxexercise groupsignificantly higher （P <0.05）;70%VO_2maxexercise group,75%VO_2maxexercise group wassignificantly higher than85%VO_2maxexercise group, P <0.05);65%VO_2maxexercise group,80%between VO_2maxThe exercise group was significantly higher than the90%VO_2maxexercisegroup （P <0.05）.3.6in each group of the succinate dehydrogenase activity in rat quadriceps changesSuccinate dehydrogenase （SDH） activity,65%VO_2maxexercise group,70%VO_2maxexercisegroup,75%VO_2maxexercise group,80%of VO_2maxexercise group very significantly higher （P<0.01）;85%VO_2maxexercise group90%of VO_2maxexercise group significantly higher （P <0.05）;75%VO_2maxexercise group was significantly higher than the85%VO_2maxexercise group,90%VO_2maxexercise group （P <0.01）;75%VO_2maxexercise group was significantly higherat65%VO_2maxexercise group,70%VO_2maxexercise group,80%of VO_2maxexercise group （P <0.05）;65%VO_2maxexercise group,70%VO_2maxexercise group,80%of VO_2maxexercise group wassignificantly higher than the85%VO_2maxexercise group,90%VO_2maxexercise group （P <0.05）;65%VO_2maxexercise group,70%VO_2maxexercise group,80%VO_2maxexercise group threegroups no significant difference.3.7in each group of rats quadriceps Ca²⁺-Mg²⁺-ATPase activity changesRat quadriceps Ca²⁺-Mg²⁺-ATPase activity,65%VO_2maxexercise group,70%VO_2maxexercise group,75%VO_2maxexercise group,80%of VO_2maxexercise group was significantlyhigher （P <0.01）;85%VO_2maxexercise group,90%VO_2maxexercise group was significantly higher than that in the control group （P <0.05）;75%VO_2maxexercise group was significantlyhigher than the85%VO_2maxexercise group,90%VO_2maxexercise group （P <0.01）;75%betweenVO_2maxThe exercise group significantly higher than the65%VO_2maxexercise group,70%VO_2maxexercise group,80%VO_2maxexercise group （P <0.05）;65%VO_2maxexercise group,70%VO_2maxexercise group,80%of VO_2maxexercise group was significantly higher than85%VO_2maxexercisegroup,90%of VO_2maxexercise group （P <0.05）.4.Conclusion4.1With the advent of exercise stress in rats increases body weight of rats significant reductionof body weight of rats in the control group,90%of body weight of rats the lowest long-term highintensity exercise exercise-induced fatigue, illness and injury in rats led to: reduced food intake,exercise a lot of energy, making the rats own composition substances catabolism, body weightgradually decreased trend.4.265%between VO_2max,85%of VO_2max, moderate-intensity treadmill training did notsignificantly increase the content of the rat quadriceps muscle glycogen, and70%betweenVO_2max and75%VO_2max intensity long-term training can significantly increase rat quadricepsmuscle glycogen content.90%of VO_2max, long-term treadmill training is not conducive to ratskeletal muscle glycogen reserves.4.3different intensity treadmill training can significantly increase the rat quadriceps aerobicmetabolic enzyme activity of SDH, MDH,90%of VO_2max, long-term treadmill training canimprove aerobic metabolism enzymes of rat quadriceps activity, this could be a compensatorybody for sports injuries.4.4different intensity treadmill training can significantly improve the anaerobic metabolism ofthe rat quadriceps enzyme LDH activity,90%of VO_2max long-term treadmill training can makethe rat quadriceps muscle glycogen, anaerobic glycolysis energy supply.4.565%between VO_2max,70%between VO_2max,75%between VO_2max,80%betweenVO_2max and85%VO_2max long-term treadmill running can significantly improve the ratquadriceps Ca2+-Mg2+-ATPase activity,90%of VO_2max, long-term running the trainingleads to skeletal muscle disorders, is not conducive to the Ca2+-Mg2+-ATPase recovery.