Epithelial Ovarian Cancer Tissues Atf - 5 And The Bcl - 2 Gene Expression And Its Clinical Significance

Abstract Objective:1. To investigate the expression and significance of activating transcription factor5(ATF-5) in epithelial ovarian carcinomas.2. To investigate the expression and significance of bcl-2in epithelial ovarian carcinomas.3. To investigate the relationship between ATF-5and bcl-2expression in epithelial ovarian carcinomas.Methods:1. The expression of ATF-5and bcl-2mRNA were examined by reverse transcription polymerase chain reaction (RT-PCR) and ATF-5and bcl-2protein were detected by immunohistochemistry in the specimens of60cases of epithelial ovarian carcinomas,15cases of ovarian benign tumors and9cases of normal ovarian tissues.2. The relationship between ATF-5and bcl-2expression and clinicopathological parameters are analyzed.Results:1. The expression of ATF-5was significantly higher in ovarian carcinomas than that in ovarian benign tumors and normal controls (P<0.05); Over-expression of ATF-5was related to the differentiation and clinical stage, the positive rates of ATF-5were notably higher in stage Ⅲ～Ⅳ than stage Ⅰ～Ⅱ (P<0.05); the positive rates of ATF-5were also higher in poor differentiation group, but there is no difference between well and moderate differentiation group(P>0.05); and there is no relationship between ATF-5expression and histological type and the patient’s age(P>0.05).2. The expression of bcl-2was significantly higher in ovarian carcinomas than that in ovarian benign tumors and normal controls (P<0.05); Over-expression of bcl-2was related to the differentiation and clinical stage, the positive rates of ATF-5were notably higher in stage Ⅲ～Ⅳ than stage Ⅰ～Ⅱ (P<0.05); the positive rates of bcl-2 were also higher in poor differentiation group, but there is no difference between well and moderate differentiation group(P>0.05); and there is no relationship between bcl-2expression and histological type of ovarian cancer and the patient’s age(P>0.05).3. There is a connection between ATF-5and bcl-2expression in epithelial ovarian cancer (r=0.461,P<0.05).Conclusion:ATF-5and bcl-2are over-expressed in epithelial ovarian carcinomas. The relative expression level of ATF-5and bcl-2is related to the differentiation and clinical stage, which lays a foundation to the further research on its relation to the origin of ovarian cancer and its role in biological behaviors of ovarian carcinomas.