| Aim:Deer skin glue was prepared as described previously in the patent, and theninvestigated effects of deer skin glue on sexual hormones, sexual organ index andendothelial nitric oxide synthase and phosphodiesterase-5 expressions in castrated andnormal rats.Methods:Deer skin glue was prepared. Forty-two male Wistar rats were randomized intoseven groups, two castrated groups (C2,L0) and five normal groups (C1,L1,L2,L3andA). The rats from castrated groups were underwent bilateral orchiectomy. One weekafter surgery, the rats were treated with normal water (groups C1and C2), oral deerskin glue (0.1g/[kg·day] for L1 group, 0.2 g/[kg·day] for L2 and L0 groups and 0.4g/[kg·day] for L3group) and intramuscular injection of Testosterone Propinas (5mg/[kg·day] for A group) for ten days. 24hours after the treatment, the weight of testis,penis, seminal vesicle-prostate, sexual hormones including serum testosterone(T) andluteinizing hormone (LH),the expressions of eNOS and PDE5 were evaluated.SPSS18.0 was used for the statistical assay. T-test was used to compare theexperimental and control groups. All the data were expressed as a mean±standarddeviation (SD) .Results:1) Sexual Hormones AssayThe mean levels of testosterone and luteinizing hormone descreaced significantly incastrated rats，while fed with deer skin glue, the mean level of testosterone(0.6±0.24mg/ml) and the level of LH (0.23±0.13mIU/ml) increased significantly(P>0.01) coparing with the level of rats (0.3±0.22 mg/ml；0.14±0.13 mIU/ml) fromgroup C2. In group L1, the levels of testosterone and luteinizing hormone were0.6±0.24mg/ml and 0.23±0.13mIU/ml. There was no significance with control group(0.3±0.22 mg/ml；0.14±0.13 mIU/ml). However, it was of statistical significances ingroup L2(4.03±0.43 mg/ml；0.53±0.13 mIU/ml) and L3 (4.97±1.87 mg/ml；0.53±0.13 mIU/ml)(P>0.01).2) Sexual Organ Index AssayThe penis index, seminal vesicle and prostate index decreased after the bilateralorchiectomy, The index increased when the rat given deer skin glue, though there wasnot statistical significance. At the same time, the sexual organ index of the normal ratsgiven deer skin glue increased too, but it was of statistical significance only inseminal vesicle and prostate index (the mean value was 6.99±0.86(mg/g) in rats fromL3,comparing with the mean value was 5.85±1.12(mg/g) in rats from C1; P < 0.01).3) Effects of Deer Skin Glue on eNOS and PDE5 ExpressionsThe expressions of eNOS and PDE5 in the rats uderwent bilateral orchiectomydecreased a lot , while fed with deer skin glue, the expression of eNOS (0.29±0.09)and PDE5 (0.24±0.04) recovered markedly (P>0.05) compared with that in C2 (0.21±0.04; 0.2±0.03).In normal groups, the eNOS expression increased significantly compared withcontrol group ( 0.58±0.16) (0.76±0.09 in group L1, P>0.05; 0.79±0.17, in groupL2 and 0.86±0.18 in L3, P>0.01). While the PDE5 expressions increased remarkably(P>0.01) in all groups fed with deer skin glue(0.63±0.11 in group L1; 0.9±0.26 ingroup L2; 1.04±0.05 in group L3) compared with control group (0.4±0.01).Discussions:The levels of sexual hormones could be increased, the expressions of eNOS andPDE5 could be enhanced, and the sexual organ index could be enhanced in bothnormal rats and castrated rats fed with deer skin glue. It implyed that deer skin gluemay have aphrodisiac function. |
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