Secondary Bone Marrow Fibrosis Of The Marrow Tissue Morphological Characteristics And Clinical Research

Objective: 1..To explore the differences in clinical features and bone marrow morphologybetween secondary myelofibrosis and primary myelofibrosis.2. To observed clinical and bone marrow pathology characteristics in patients withhematologic malignancies with secondary myelofibrosis by retrospective analysis.Methods: 1.Patients:Into this retrospective study 105 patients (52 males，53 females) wererecruited between 2007 and 2011 of the Department of Pathology in Hospital ofBlood diseases, including 69 patients with secondary myelofibrosis and 36 caseswith primary myelofibrosis .2. Bone marrow biopsy specimens：Bone marrow biopsy specimens were obtainedand processed according to standard procedures.Representative trephine biopsies ofthe bone marrow(mean size 2.0mm×10.0mm)were performed from theposterioriliac crest in all patients. Bone marrow biopsy plastic-embedded sectionswere stained with H-E and Gomori’s.3.To observe and evaluate newly the histopathological features of bone marrowbiopsy specimens with 105 cases about，then did statistic analysis，including theproliferation degree of bone marrow tissue，the hyperplasia extent ofgranulocytic-lineage，erythrocytic-lineage，megakaryocytic-lineage，morphologyand number of megakaryocytes,then make correlation analysis of bone marrowfibrosis and the number of megakaryocytes.4．The statistical analysis of the clinical features and bone marrow smear morphologyon 105 patients．5.The experimental data were statistically analyzed using SPSS 13.0.Results:1. The age of SMF was significantly lower than this in PMF(P＜0.05).2.The detection rate of mild anemia was significant difference between SMF andPMF(P＜0.05). The degree of proliferation of bone marrow smears and bone marrowbiopsy of two groups were significant differences(P <0.05) .With Gomori’s stains ,the levels of the reticular fiber detection rate was significantdifference (P <0.05) in bone marrow biopsy.3.There did not have a significant corrrelation between bone marrow fibrosis and thenumber of megakaryocytes in patients with SMF.Conclusion:1. Various clinical diseases of blood system could associated with secondarymyelofibrosis,especially Myeloproliferative neoplasms.2.SMF occurred earlier than PMF ,and it was depending on the age of theirunderlying disease. 3.Compare with PMF,the degree of proliferation was more hyperplasia in SMF,it isimportant to find the original cause of myelofibrosis in young patients.4.Megakaryocytic cells is an important possible factor in cause of primarymyelofibrosis.In secondary myelofibrosis ,megakaryocytic cells and multiplecytokines which released by it megakaryocytic cells remains to be further studied.