Striped Hamsters Ppar Beta Cloning And Snp Analysis Part Of The Sequence

Cricetulus barabensis，wide distribution, the destructive wait for a reason, be in the north ofthe advantage of the farmland against one of the rat. Begin with breeding genes, from molecularlevel reveals harmful rat population fluctuation mechanism, provide new ideas forcomprehensive prevention and control the rat. This paper is mainly on the reproducive genePPARβ for the molecular biology study.PPARβ gene (Peroxisome proliferator-activated receptors beta) as one of the nuclearreceptor superfamily members, already appeared as early as in an early stage in embryonicdevelopment, and has close relationship with animal embryo implantation, the formation of theplacenta, the embryonic development, the maintenance of ovarian function, in addition inindividual growth process, PPAR β distribution is quite widespread and has important regulatoryrole to the normal growth and development of the individual. This is the first time amplify thePPAR β gene sequence of C.barabensis by cloning method, and made single-nucleotidepolymorphisms (SNPS) analysis in part sequence of C. barabensis, to provide the theory basis atthe molecular level for the C. barabensis.This study to capture in shandong province C. barabensis as the research object, withRT-polymerase chain reaction (PCR) cloning get PPAR β mRNA exon4-6part of the sequence480bp (GenBank registration number: JN662960), and make the biology analysis and forecastthe nucleotide, amino acid sequence, secondary structure and space structure. In its structureanalysis process found the special structure area: zinc finger structure. In the secondary structureof protein Alpha helix of accounting for a large scale, but zinc finger structure is located in thesmaller hydrophobic areas. To make the homology compared the corresponding PPAR β mRNAsequence of C. barabensis with Mesocricetus auratus, Mus musculus, Rattus norvegicus, Susscrof, Bos Taurus, Oryctolagus cuniculus,et al. and make the system evolutionary tree bydifferent methods. Results show that the C. barabensis has closest genetic relationship withMesocricetus auratus, Mus musculus, Rattus norvegicus, and further with Sus scrof, Bos Taurus,Oryctolagus cuniculus, and furthest with Homo sapiensc, this result with more species oftraditional classification relationship between evolution is consistent, explain PPAR beta can beevolutionary relatively conservative.With the first time use PCR method get the PPAR β gene297bp of thirty C.barabensis inShandong province, including exon6(175bp), introns6(104bp) and exon7（18bp）.UseDnaSPv5.0software analysis found that, in this section, the number of Haplotypes:11; Variable(polymorphic) sites:10, among them, there are6in the exon6, and4in the intron. This sectionanalysis found that conversion has9; Transversion is1. PPAR β gene exon area the probability of mutations bases is larger, the cause of the amino acids encoding corresponding change. PPARβ gene rich SNP offers possibility for population genetic diversity and species can better adapt tothe external environment.