Study Of The Oxidative Stress Pathogenesis Of Parkinson’s Disease(PD) Rat Modeis In The Neurons

Objective:To explore the oxidative stress pathogenesis of Parkinson’S disease(PD) rat models in the neurons.Methods:Hemi-Parkinsonism rat model was established by stereotaxic 6-OHDA lesions in substantia nigra, with apomorphin (APO)-induced rotational behavior conducted to assay the quality of the model after one week. observed the morphological alterations by striatal and substantia nigral hematoxylin & eosin (HE)stain and compared the quantities of No2-(the metabolism products of nitric oxide)between the injuried tissue and normal tissues. Observe the Level of inos and Tunel in the right substantia nigra(SN)of the model group through immunohisto chemical stain。Results:In the m odel group of 30 rats, there were 21 successful PD models. the quantity of No2-in the right substantia nigra (SN)of the model group increased obviously respectively compared w ith either the sham group or the normal control group (P< 0.05). Photom icrographs of HE stain showed that the dopam inergic neurons in the right substantia nigra pars compacta (SNc) were severely lost, and the remaining neurons were atrophic. the Level of inos and Tunel in the right substantia nigra (SN)of the model group increased obviously。Conclusion:It is rather effective method to make PD rat models using 6-OHDA injected into the striatium of one side at two target sites. In PD rats the quantities of No2-are increased in the dopaminergic neurons of SN. the Level of inos and Tunel in the right substantia nigra (SN)of the model group increased obviously. oxidative stress plays a key role in the pathogenesis of PD.