The Expression Of Doc2/dab2and C-FOS In Laryngeal Squamous Cell Carcinoma And Its Clinical Indication

Objective：To investigate the expression of Doc2/dab2and C-FOS inlaryngeal squamous-cell carcinoma, the lympatic node metastasis andnormal laryngeal squamous epithelium and to study the role of Ras/Mapkpathway in laryngeal carcinogenesis, development and metastasis in order toprovide theoretical basis to laryngeal cancinoma therapy.Methods: The expression of Doc2/dab2and C-FOS were evaluated bySP immunohistochemical method in40cases of laryngeal squamous cellcarcinoma,13cases of lympatic nodes metastesis,30cases of normallaryngeal squamous epithelium. The relationship between the expression ofDoc2/dab2and C-FOS and the clinical factors was analyzed.Results:1The expression of Doc2/dab2protein in laryngeal squamouscell carcinoma tissues: The immunohistochemical study indicated that thepositive staining of Doc2/dab2protein was seen mainly in cytoplasm. Thepositive expression rate of Dab2/dab2in normal laryngeal squamous cell,laryngeal squamous cell carcinoma and metastatic lymphatic node was93.33%,62.50%and30.77%, respectively. There was significant differenceamong these groups (P<0.05). The positive expression rate of Doc2/dab2inwell differentiated laryngeal squamous cell cancinoma was76.00%and thepositive expression rate in moderate-poorly differentiated laryngeal squamouscell cancinoma was40.00%. The positive expression rate of Doc2/dab2inlympatic nodes with metastesis was30.77%and positive expression rate was66.67%in lympatic nodes without metastesis. There was significant differencebetween these two different tissues (P<0.05). The expression of Doc2/dab2was related to pathological differentiation degree and lympatic nodesmetastesis, but was not related to patient age, tumor position, TNM stage andclinical stage (P>0.05). 2The protein expression levels of C-FOS in laryngeal squamous cellcarcinoma tissue: The immunohistochemical study indicated that the positivestaining of C-FOS protein was seen mainly in cell nucleus or cytoplasm. Thepositive expression rate of C-FOS in normal laryngeal squamous cell,laryngeal squamous cell carcinoma and metastatic lymphatic node was36.67%,65.00%and100%, respectively. There was significantly differentamong thesr groups (P<0.05). The positive expression rate of C-FOS in welldifferentiated laryngeal squamous cell cancinoma was52.0%and the positiverate was86.67%in moderate-poorly differentiated laryngeal squamous cellcancinoma. There was significant difference between the different cancertissues (P<0.05). The positive expression rate of C-FOS in lympatic nodeswith metastesis was100%and48.15%in lympatic nodes without metastesis.There was significant difference between the two different tissues (P<0.05).The expression of C-FOS was related to pathological differentiation degreeand lympatic nodes metastesis, but was not related to patient age, tumorposition, TNM stage and clinical stage (P>0.05).3The relationship between the expression of Doc2/dab2and C-FOS: BySpearman Correlation analysis, data showed the expression of Doc2/dab2wasnegative related with the expression of C-FOS（r=-0.0352,p=0.026）.Conclusions:1The expression of Doc2/dab2in laryngeal squamous cell cancinomawas obviously lower than that in normal laryngeal squamous cell tissues. Theexpression of C-FOS in laryngeal squamous cell cancinoma was obviouslyhigher than that in normal laryngeal squamous cell tissues.2The expression of Doc2/dab2and C-FOS was related to pathologicaldifferentiation degree and lympatic nodes metastesis, but was not related topatient age, tumor position, TNM stage and clinical stage.3Laryngeal squamous cell cancinoma with low expression of Doc2/dab2was apt to metastasis.4Doc2/dab2may repress the cancer metastasis not only through MAPKpathway but also through other unknown pathway.