| Objective： Antibiotic associated diarrhea refers to the use of antibioticsthat causes intestinal flora disturbance caused by diarrhea. Secondarydiarrhea occurred not only increased the patient’s condition and increase inother types of the risk of hospital acquired infection, but also extended thelength of hospital stay, all above increase the patient’s medical treatment cost.Moreover, intestinal flora disturbance brings several side effects to body’simmune response and physiological metabolism. So early treatment isnecessary to improve the prognosis, produce good social and economicbenefits. Diamine oxidase (DAO) mainly distributed in the intestinal tract,located mostly in the top of intestinal mucosa in cells and peripheralblood,which activity changes could reflect the intestinal mucosal barrierfunction. When the intestinal mucosa damaged, DAO enter within the bowellumen intercellular lymphatic vessels and capillaries, increased levels of DAOin the blood. In this experiment, according to measuring diamine oxidase(DAO) in the plasma before and after children suffered with secondarydiarrhea applied the probiotics,the therapeutic effect of probiotics toasthmatic pneumonia in children with secondary diarrhea were evaluated.Method： AAD children suffered from asthmatic Pneumonia wererandomly divided into two groups，group A and group B.Two groups weregiven antibiotics and relevant treatments. Group A was given montmorillonitepowder (trade name: close to, bo fu-good quality pharmaceutical co, LTD.,production, approval number: H20000690, approved by the <1year old0.3package every time, every time~2years old0.5package,~3years old,1bageach time, both orally3times a day) Group B was given montmorillonitepowder on the basis of saccharomyces boulardii sachets powder (trade name: live, encyclopedia of pharmaceutical production, France Jin Kou Yaoregistration number S20100086, three years of age or older children:1bageach time,2times a day. Children under the age of3:1bag each time,1timesa day) the diarrhea situation (stool properties and number) of children,duration of diarrhea, total in hospital treatment and application of probioticswere observed. Children with diarrhea were detected including stool routine,bacterial culture and rotavirus stool antigen detection. Curative effect ofstandard: according to the standard which was formulated on the conferenceabout diarrheal disease prevention in1998.The curative effect was ranked into3groups: Obvious：the times and the characters of defecate returned to normalstation within72h after treatment, and the systemic symptom disappeared；Effective: the times and the characters of defecate improved significantlywithin72h after treatment；Invalid: the times and the characters of defecatehad no improvement and even the children dead. Effective and efficientscheme were the total effective rate. And the plasma of two groups childrenwere collected at the different time before and after diarrhea (Prior to diarrhea,the third day and a week after diarrhea). Plasma samples for the DAO level ismeasured by using enzyme-linked immunosorbent test method andspectrophotometer colorimetric method. Statistical analysis was performedusing SPSS13.0software package.According to nature the data are dividedinto count data and measurement data. Statistical description: if measurementdata conforms to normal distribution with mean±standard(x S) deviation,unnormal distribution with the median (interquartile range), the count datawith(%). Statistical analysis： Homogeneity of variance with the t test, ifvariance is not homogeneous with nonparametric test (Mann-WhitneUinspection), count data by chi-square test. Compare the results with P <0.05for the difference was statistically significant.Results:1Two groups of children with clinical data: two groups of children with67cases, the treatment group (35cases), male (19cases) female (16cases); Control group (32cases),17were male(17cases), female (15cases).Differences compared among the two groups of gender, age, weight,et al. The result of the analysis is they have no statistical difference (P>0.05).2The duration of diarrhea between the two groupsThe duration of diarrhea of group B was longer than that of group A[5.00(2.00) days vs3.50(1.00) days, P<0.01].3The total length of hospital stay between the two groupsThe total length of hospital stay of group B was shorter than that of groupA [9.00(2.00) days vs10.00(3.00) days, P<0.01].4Curative effective rate between the two groupsThe curative effective rate of group B was higher than that of group A(91.43%vs68.75%, P<0.05).5Plasma DAO level changes between the two groupsBefore diarrhea: There was no difference between group B and group A inthe plasma DAO level (9.789±1.312U/ml vs.10.039±1.114U/ml, P>0.05).Three days after diarrhea：The plasma DAO level of group B was lowerthan that of group A [31.741(2.952)U/ml vs.50.350(4.175)U/ml, P<0.01].Seven days after diarrhea：The plasma DAO level of group B was lowerthan that of group A (14.711±1.367U/ml vs.19.693±1.288U/ml, P<0.01).Conclusion:1Unreasonable use of antibiotics can cause intestinal flora imbalance, leadto antibiotic associated diarrhea.2Early application of probiotics (billion live) can shorten the diarrheaduration and total length of hospital stay of pneumonia childrenbrought the maximum protection of the body, reduced the impact on thestructure of intestinal flora, protect the intestinal mucosal barrier structure,improved the prognosis, and reduced the family economic burden.3Compared with the control group, combined application of probioticshad better curative effect. 4Both the DAO level in the plasma of the treatment group and controlgroup after the third day and a week were higher than pre-diarrheaDiamine oxidase can reflect the early state of diarrhea in children withgastrointestinal mucous membrane barrier function. |
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