Protective Effects Of Ulinastatin On Heart Of Rats With Acute Myocardial Infarction

Acute myocardial infarction (AMI) is a kind of serious impact on humanhealth. Because of it, which is one of the most important fatal diseases in thecardiovascular system, the function of the heart is reduced greatly. Operationis stimulation in patients with cardiovascular disease. In these patients the riskof getting AMI is highly increased. The management of surgery or emergencytreatment on patients who had a history of cardiovascular disease or getintraoperative AMI has become especially important responsibility to eachphysician and anesthesiologists. The protection of vital organs must be payedespecially attention. Protect the heart function after acute myocardialinfarction and thus become an important research topic.Objective: This experiment selected in rat model of acute myocardialinfarction to discuss the effect of ulinastatin on myocardial apoptosis andcardiac function after acute myocardial infarction. Provide theoretical basis fortreatment of acute myocardial infarction in patients.Methods:30healthy male SD rats, were randomly divided into3groups:sham operation group, control group and ulinastatin group,10rats in eachgroup. The control group and ulinastatin group rats with thoracotomy threadligation the left anterior descending coronary artery to establish acutemyocardial infarction model; sham group rats thoracic and threading in theventricular wall without ligation of the left anterior descending coronary artery.Rats in control group and sham group were given intraperitoneal injection ofNS1ml per day for3days. Ulinastatin group rats were given intraperitonealinjection of ulinastatin80000U/kg in postoperative treatment for3days. Therats were given penicillin intramuscular injection400000U/kg in thepostoperative to prevent infection. In each group5of the10rats were detectedthe myocardial cell apoptosis rate by flow cytometry one week after the surgery. The rest5rats would get heart function detection4weeks after thesurgery. Ventricular dp/dt max(±dp/dt) and the heart rate(HR) would bedetected.Results:1The rate of myocardial cell apoptosis was detected by a flow cytometry.Ulinastatin group rats after operation (25.40±1.91%) than the control rate ofmyocardial cell apoptosis after one week after operation in group (43.23±4.40%) significantly reduced, and this reduction was statistically significant(P<0.05).2.1Result of heart function4weeks after the surgery.2.2The rate of+dp/dtmax systolic pressure of left ventricular circumferencemaximum variation in ulinastatin group rats after operation (4204.25±602.16),left ventricular diastolic pressure (maximum change rate of-dp/dtmax was4169.17±711.54) than in the control group rats were average values of+dp/dtmax (3558.41±349.08)-dp/dtmax,(3380.85±249.90) have increased,but there was no statistical significant differences (P>0.05).2.3Rats in ulinastatin group after operation (360±12) around the heart rateand the sham group (341±21), no significant difference (P>0.05). The rats incontrol group after operation (392±15) around the heart rate than theulinastatin group and sham group increased, the difference was statisticallysignificant (P<0.05)3Oberservation of HE stain: the sham group myocardial tissue arranged inneat rows, few inflammatory cells; tissue edema and hyperemia of the controlgroup, and a large number of inflammatory cells infiltration; the ulinastatingroup edema, inflammation cells infiltration was significantly less than that ofthe control group.Conclusions: The therapy of ulinastatin on acute myocardial infarction inrat heart has certain protective effect. Ulinastatin can be used to protect theheart function in patients who get acute myocardial infarction during thesurgery.