Serum And Urine Of Patients With Primary Glomerular Disease Mouse Podocyte Synaptopodin And Podocalyxin Expression Effect

Objective: Experiments in this paper using podocyte culture technologyto research from the cellular level of IgA nephropathy minimal lesions inpatients with membranous nephropathy in mice serum and urine podocyteSynaptopodin and Podocalyxin expression, and further explore the podocytespecificity molecular relationship with proteinuria, facilitate clear podocytespecificity molecular relation with primary glomerular disease severity, whichis new in the diagnosis and treatment of primary glomerular disease path.Background: recent study found that minimal lesions in membranousnephropathy IgA nephropathy diabetic nephropathy with lupus nephritis andcrescent nephritis onset or progression of podocyte injury may be pathogenickey, the key is likely to be sufficient cell surface marker protein abnormalexpression of cell surface marker abnormal podocyte surface marker proteinexpression can be directly or indirectly affect normal cell structure changes,lead to dysfunction of podocyte of normal structure glomerular podocyte losseventually lead to a variety of glomerular disease glomerular podocyte as itsname implies is part of the glomeruli, are dirty glomerular layer of epithelialcells, are highly differentiated epithelial cells of the mature, is to have largevolume, forms of diversity, complex cells by the function and structure ofdifferent cell body foot process (FP) sudden three parts between adjacent thefoot processes of about25nm in diameter, the thickness is about4-6nm, like apull chain fracture membrane (SD) cover near the side of glomerular basementmembrane (GBM), is the outermost layer of the glomerular filtrationbarrier.When the glomerular filtration barrier permeability increase will haveprotein leakage glomerular podocyte foot process can be divided into threefunctional different membranous structure roof membrane area in the diaphragm area (SD) basement membrane zone (connected with GBM) footprocess containing like podocin nephrin actin podocyte proteins such as footskeleton of actin cytoskeleton proteins have been shown the features of thecapillary loops inherent spatial structure stability; To maintain podocytestructure of dynamic and static; The glomerulus internal pressure resistance;Synthesis and secretion of vascular endothelial growth factor; Maintainintegrity of glomerular endothelial cells function; And the composition of theplasma protein molecular charge filtration barrier In addition also containsis unclear the bioactive factors derived cytokines of endocytosis and changes inrecent decades found enough cells the expression of specific protein moleculesby region can be divided into: SD protein complexes (Nephrin and itshomologue, PodocinCD2related proteins, TRPC6; classic element calciumgummy (P-cadherin) the original glue calcium, FAT, tight junction protein ZO-1, etc.) are connected to the GBM basement membrane zone protein (A3B1integrin; Dystroglycans) roof area membrane protein (such as Podocalyxin;glomerular epithelial protein-1) actin related protein (Synaptopodin, A-actinin-4, Ezrin) Tumor relevant protein (WT1).Sertoli cell expression ofspecific proteins expression situation directly or indirectly affect thedevelopment and prognosis of kidney disease.Method: The primary glomerular disease cases of the experiment for30cases (including10cases of IgA nephropathy, minimal lesions in10cases,10cases of membranous nephropathy) of serum and urine to return: March2011to December2012, selected a diagnosis of10cases of idiopathic membranousnephropathy patients, hospitalized in hebei medical university second hospitalurology department parallel clinical and pathological confirmed renal biopsy,including hepatitis screening negative markers, autoantibody negative,negative, is not taking hormone and immune inhibitors and kidney protectionconsists of ACEI or ARB drugs. Except for hypertension, diabetes, hereditarynephritis. The clinically characterized by quantitative>24h urine protein3.5g of patients in5cases, normal serum creatinine, eGFR>90ml/min. PrimaryIgA nephropathy patients serum and urine: in March2011to December2012, ten patients in the second hospital, hebei medical university renal medicinehospital parallel clinical and pathologic diagnosis of renal biopsy of the lgAnephropathy, except allergic purpura, systemic lupus erythematosus (sle),psoriasis, cirrhosis of the liver, etc., not used hormone and immune inhibitordrugs contain ACEI or ARB and kidney protection, except high bloodpressure, diabetes, hereditary nephritis. But clinical quantitative>24h urineprotein1.0g <2.0g, serum creatinine were normal, and eGFR>90ml/min.Primary glomerular minimal lesions: in March2011to December2012)diagnosed with small lesion in second hospital, hebei medical university renalmedicine renal biopsy in patients with clinical and pathological confirmed10cases, except such as heavy metal poisoning cause, not used hormone andimmune inhibitors and renal protection of drugs including ACEI or ARB orstatin lipid-lowering, except high blood pressure, diabetes, hereditary nephritis.24h urine protein quantitative of10cases were clinically>3.5g, normalserum creatinine and eGFR>90ml/min. Different concentrations of serumand urine in12,24and48h stimulate the in vitro cultured mouse sertoli cellline immunocytochemistry staining. Through pathological image analyzer todetect Podocalyxin and Synaptopodin index expression intensity.Results: Immunocytochemistry, according to the results of minimallesions in group IgA group concentration is5%of serum and10%of urine ofSynaptopodin expression effect is within48hours of expression intensityincreased with the extension of time, the control group no significant change;And IgA minimal lesions in group concentration is5%serum and10%ofurine of Podocalyxin expression effect is the extension of time within48hoursand fell, the control group no significant change; In membranous nephropathygroup concentration is5%serum and10%of urine of Synaptopodin expressionis affected within48hours to24hours from time to tome brief rise after thefall of the control group no significant change. In the membranousnephropathy group concentration is5%serum and10%of urine Podocalyxinimpact is still within48hours longer expression gradually decreases with time,the control group no significant change (P <0.05) in blood and urine stimulate cells detect Podocalyxin group form thin, cell number is less; Cell number andform changes and tests Synaptopodin group were not compared amongdifferent types of kidney disease, Synaptopodin or Podocalyxin expression ofno statistical significance (p <0.05)Conclusion: After through stimulation of glomerular podocytePodocalyxin and Synaptopodin surface marker protein expression changes,show specific molecules expression abnormal on the impact of different typesof primary glomerular disease development difference is not statisticallysignificant, expressed in a single type of disease affects obviously trend andhas statistical significance. In different types of proteinuria in kidney diseasefailed to detect the effect of this experiment, need further study.