CD207Expression In Vitiligo And Halo Nevus

BackgroundAutoimmune diseases(AID) refers to the immune system by environmental or genetic factors that suffered, which damage imbalance produces antibodies against its own tissue components and lymphocytes, damage to its own tissues and organs, leading to disease. The disease often in clinical performance, has been a long time ago asymptomatic prodromal period. Such diseases can be divided into specific and systemic autoimmune disease, the former, such as rheumatoid arthritis (RA), multiple sclerosis (MS), Type1diabetes mellitus (IDDM), Systemic lupus erythematosus (SLE).Systemic lupus erythematosus belong to systemic autoimmune disease According to statistics, the autoimmune disease crowd incidence of5-10%, about80different manifestations, morbidity and mortality is the highest one of the diseases. International called "5D" disease that discomfort, disability, drug intoxication,dollar loss, death. Autoimmune diseases are more common in women, such as systemic lupus erythematosus, the incidence of women about male9-10times, but a small part of males more than woman, such as the ankylosing spondylitis. Autoimmune pathogenesis of the disease is quite complex, and the concrete is not yet entirely clear.Vitiligo is a disease that skin and mucosal pigment cell destruction due to the depigmentation disease. The global incidence of at least1%to2%, about0.1%-2.7%of the incidence of the Chinese people. Divided into two types according to the vitiligo latest typing methods. Two types:vitiligo vulgaris (solitary, scattered, generalized, acromegaly) and stage, the former is also known as non-stage type and this type of vitiligo patients are more sensitive to external pressure and friction, and its incidence of90%; Two categories:completely leukoplakia and incomplete leukoplakia; two stages:advanced and stable period. The melanocytic lesions exact reason is not clear, but a large number of observers believe cell-mediated immunity playing an important role. such as the1977, Hertz and others found in the type of patient serum IgG antibodies that can be combined with the melanocytes in the skin biopsy and put forward the concept of "autoimmune vitiligo".Halo nevus,known as the eccentric acquired white, faded spots around the limitations of pigment stains. Some people think that the disease is a special type of vitiligo and occured with vitiligo together, there is no unified reported incidence. Halo nevus of statistics1%-47.8%of patients with vitiligo;0.5%-50%of patients with vitiligo associated with halo nevus. Typical halo nevus in four phases, halo nevus serum anti-melanocyte antibodies, and antibody titers disease activity. Liu Zhuo and other studies have shown that halo nevus white spot at the CD4+decrease in the number of T cells, CD8+T increased,peripheral blood CD4/CD8ratio decreased and associated with disease activity. The halo nevus histopathological:nevus cells and their surrounding a large number of lymphocytes, part of macrophage infiltration, nevus cells and melanocytes are destroyed.Langerhans cells (LC) is one of the bone marrow-derived dendritic cells, and is the primary line of defense against external antigens distributed in parts of the basal layer over the skin and mucous membranes, which is an immature dendritic cells, the royalty cells as professional antigen in the skin immune system play an important role. Its gene is located on chromosome2p13, acrossing5.6kbp and including six exons, except the first intron splice sites in GT-AG-II. Gene upstream region does not contain a consensus promoter elements.CD207/langerin is a specific marker of Langerhans cells, its chromosome positioning:forward5’-ACGCCATTCAACAAG GTC, reverse,5’-CAGCCAAG ACAGACGGACTC, which is the type Ⅱ transmembrane C-type lectin superfamily protein. CD207does not belong to any known C-type lectin gene cluster and can induce the formation of Birbeck granules. It can promote carbohydrate antigen endocytosis and antigen presenting, and play an important role in the antigen-presenting. Study found that langerin-positive cells of MHC II also positive, but we have observed rarely langerin-positive cells, speculated that most of the LC cells in transport state.1992Caux and other applications of CD34+hematopoietic stem cells with GM-CSF (granulocyte-macrophage set off the stimulus factor) and of TNF-a (a tumor necrosis factor) culture, differentiation for the two types of sub-groups:CD14+CD1a’and CD14+CD1a+.The former express Langerin/CD207The domestic has only one downregulated CD207in condeylomata acuminnatum (CA)report, but haven’t CD207expression in vitiligo skin and halo nevus. Abroad less abnormal expression of CD207in vitiligo skin and halo nevus. Thus study CD207expression in different types of vitiligo lesions and halo nevus, will play an important role for the further study of the pathogenesis of vitiligo and halo nevus.ObjectiveStudy CD207expression in different types of vitiligo lesions and halo nevus and a review of the LC and related cells function,.That will provide clues to further explore the cellular immune vitiligo and halo nevus pathogenesis, clinical classification, can provide different treatment for different types of vitiligo treatmentprogram.Materials and Methods1. The cases were from our hospital. Diagnosis and classification using the latest Chinese Association of Integrative Medicine of Dermatology Professional Committee pigment disease study group revised the of vitiligo clinical classification and efficacy standards vitiligo patients divided into vitiligo vulgaris and stage type. Into stage type (13cases) and vitiligo vulgaris (28cases) of the38patients with vitiligo. Halo nevus9cases.The control group of10patients, all healthy persons during the same period (both general surgical resection after more than normal skin). All patients had the disease outside of autoimmune diseases and systemic diseases, a month before the histopathology collection is not systematic or topical corticosteroids or other immunosuppressive agents. Gender and age differences among the three groups were not significant.2. Using immunohistochemical detection of CD207in13cases of stage type vitiligo patients,25vitiligo vulgaris,9cases halo nevus patients with advanced vitiligo patients,10cases of healthy expression.3. Statistical analysis:All data are represented by X±S, SPSS16.0software for data analysis by one-way ANOVA methods to deal with, the Kruskal-Wallis test;pairwise comparisons using Bonferroni test methods for testing. Pairwise comparisons using the Bonferroni method, P<0.05for the difference was statistically significant.Results1.The expression of CD207distribution Where immunohistochemistry appears black, brown or tan round, oval or irregular in shape and have a dendritic cell protrusions being recorded as the positive expression of CD207.2.CD207expression counts and statistical significanceCD207per mm in segmental vitiligo, vitiligo vulgaris expression number respectively (61.2±10.9) and (64.3±12.5), halo nevus is (75.8±16.6),normal control was (52.4±10.3), patient groups compared with the normal control groups, there was statistically significant (P<0.05), segmental vitiligo and vitiligo vulgaris compared no significant differenceConclusions1. CD207has a highly expression in various types of vitiligo lesions. This result indicates that cellular immunity be involved in the pathogenesis of vitiligo. 2.Halo nevus lesions at CD207was highly expressed.Compared with vitiligo group, the epidermis and dermis of halo nevus CD207expression were increased. Its shows that the cellular immunity of Langerhans cells are involved in the pathogenesis of halo nevus.