| ObjectiveTo observation the effects of bone marrow mesenchymal stem cells transplantation on cognitive function and expression of cell division cycle42GTP-binding protein in hippocampus of chronic cerebral ischemia rats.1To observation the expression of cell division cycle42GTP-binding protein in hippocampus of Chronic cerebral ischemia rats after bone marrow mesenchymal stem cells transplantation, speculating the effects of bone marrow mesenchymal stem cells transplantation on cell division cycle42GTP-binding protein in hippocampus.2To observe the changes of learning and memory function in rats and explore the effects of Cdc42on their memory and cognitive function.3To explore the ways of the impact of bone marrow mesenchymal stem cell transplantation on cognitive function in chronic cerebral ischemia rats.Materials and Methodology1. The150g Sprague-Dawley rats were killed by cervical dislocation. The bone marrow cavity cells of hind limb femur, tibia and were grown in DMEM-F12(10%FBS) culture spare. Portion of bone marrow mesenchymal stem cells were cultured in the culture femur containing lentivirus which carried green fluorescent protein gene. The cells of covered the bottom of the bottle were transplanted into the rats from the tail vein.2.72SD rats were randomly divided into sham group,2VO model group, the experimental group (2VO model+stem cell transplantation). The model group was taken permanent bilateral carotid artery ligation to manufacture chronic cerebral ischemia model. Sham group was as same as the model group except bilateral carotid artery ligation. Every group was set three time points of8,10,12weeks and each time point had8rats.3To detect the escape latency and the spatial memory capacity of rats by Morris water maze. To count of the positive cells of Cdc42by immunohistochemistry and detect the gray value ratio of Cdc42and β-Actin by Western blot. To analyse the trend of Cdc42protein expression and cognitive function change in rats.Results1. The expression of Cdc42protein in hippocampus of chronic cerebral ischemia rats gradually decreased with time. The immunohistochemistry displays the neuron in hippocampus of model rats degeneration and necrosis, the number of losing, and gradually increased with time (P<0.05). The number of neurons in the hippocampus of sham-operated rats was no significant differences in the three time points (P>0.05). Western blot shows the expression of Cdc42protein of sham group was no significant difference. The protein expression of model group was significantly reduced and gradually decreased with time (P<0.05). The Results of immunohistochemical and Western blot are consistent.2. The bone marrow mesenchymal stem cells transplantation can increase expression of cell division cycle42GTP-binding protein in hippocampus of chronic cerebral ischemia rats. Immunohistochemistry showed that the neuron in hippocampus of experimental group degenerated and necrosed, the degree of cell loss gradually increased with time (P<0.05). However, compared with the model group in corresponding time points, The degree of Cell degeneration and necrosis and loss significantly reduced (P<0.05). Western blot showed the protein expression of the experimental group raised compared with the model group in corresponding time points. The Results of immunohistochemical and Western blot are consistent.3. The Cdc42protein expression increase in hippocampus can improve cognitive function in rats. The test data by Morris water maze, immunohistochemistry, and western blot show:The cdc42protein expression in rat of model and experimental groups gradually reduced as the prolongation of ischemic time. The escape latency is gradually extended (P<0.05) and the across the platform number of time in a fixed period gradually reduced. Compared with the model group, The Cdc42protein expression in rat of experimental groups gradually increased in corresponding time points. The escape latency is gradually shorter (P<0.05) and the across the platform number of time in a fixed period gradually increased. The protein expression in three time points of the sham group induced expression didn’t significantly reduce learning and memory function wasn’t significant changes.Conclusion1. The chronic cerebral ischemia can affect the Cdc42protein expression in hippocampus of the rat. The Cdc42protein gradually decreases over time within a certain range of time.2. The bone marrow mesenchymal stem cell transplantation can regulate of the Cdc42protein expression in hippocampus of chronic cerebral ischemia rats. The protein expression trends to increase within a certain range of time.3. the increasing of Cdc42protein expression in hippocampus improves cognitive function in chronic cerebral ischemia rats.4. the bone marrow mesenchymal stem cells transplantation can significantly improve the cognitive function of chronic cerebral ischemia rats, which may be related to Cdc42protein expression in hippocampus of chronic cerebral ischemia rat promoted. |
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