The Distribution And Drug Resistance Of Oral Saccharomyces Spp. Among Urban And Rural Residents In Lanzhou And The Detection Of Oral Saccharomyces Albicans In Real-time Fluorescent Quantitative PCR

Objective:The purpose was to investigate and analyze the distribution of oral Saccharomyces spp. and the sensitivity to four antifungal agents among urban and rural residents in Lanzhou; So as to be helpful for the prevention, earlier diagnosis and treatment of oral candidosis. And to set up a Real-time Quantitative PCR assay with high sensitivity, specificity and rapidity to detect oral Saccharomyces albicansMethods:Concentrated saliva samples from Lanzhou resident volunteers were used for Saccharomyces spp. to be isolated and identified by CHROMagar Saccharomyces culture medium. Its susceptibility to fluconazole, amphoterincin B, nystafungin and5-flurocytosine was detected by the method of fungal susceptibility paper. Volunteers were grouped according to age, gender, living location (urban and rural) and so on.And we used Real-time Quantitative PCR assay to detect oral Saccharomyces albicans and evaluated its sensitivity, specificity and repeatability。Results:The whole detection rate of oral Saccharomyces spp. is36.31%(342/942), of which S. albicans is15.92%(150/942),S. glabrata is9.67%(91/942), S.tropicalis is9.13%(86/942),and S.krusei is3.40%(32/942) respectively. In age21-60group, calculus dentalis group, male group, denture group and urban group, the Saccharomyces spp detection rate however is higher than the controled groups in various groups (P<0.05). The sensitive of oral Saccharomyces spp. to fluconazole, amphoterincin B, nystafungin, and5-flurocytosine was61.2%,94.3%,89.6%and58.7%respectively. The resistance of oral S. albicans to fluconazole, amphoterincin B, nystafungin, and5-flurocytosine is35.7%,2.4%,4.8%and28.6%respectively. And the results obtained from the Real-time Quantitative PCR assay and the general culture were the same by detecting the samples of40cases. Its specificity was100%, and its sensitivity was100%. The lower limit of detection of this Real-time Quantitative PCR assay was10copies of recombinant plasmid DNA. The result within group and between groups were the same as the general culture.Conclusion:S. albicans is a dominant species in the oral microorganism detection among urban and rural residents in Lanzhou. Oral Saccharomyces spp. displays different resistance to four antifungal agents. The sensitive of oral Saccharomyces spp. to amphoterincin B and nystafungin is higher than others. Clinical oral candidosis therapy should be conducted along with the results of pathogen identification and antifungal susceptibility testing. The Real-time Quantitative PCR assay can detect oral Saccharomyces albicans, sensitively, specifically, rapidly, simply and stably, and will be useful to early diagnosis and target treatment of the diseases caused by Saccharomyces albicans.