The Positive Expression Of PED/PEA-15in Cardia Gland Cancer

Objetive:cardia is one of our common gastrointestinal malignancys,with a high incidence of rapid transfer, and high mortality. The five-yearsurvival rate, a serious threat to the health of our people. GCA is our mainpathological types of gastric cardia. With the continuous development ofmedical standards, including surgery, radiotherapy, chemotherapy andemerging biological treatment methods continue to improve, but it is stillnot optimistic prognosis of gastric cardia adenocarcinoma patients. Thestudy found that cell proliferation and apoptosis in a state of dynamicequilibrium, balance disorders, neoplastic diseases may occur. Inhibitionof apoptosis, the stronger the effect, the poorer the prognostic effect thefaster transfer.Condorelli et al study insulin resistance genes related to type2diabetes in1998found that the inhibitor of apoptosis protein Theexpression of PED/PEA-15was up-regulated. Involved in cell signaltransduction, apoptosis, ERK activation of other cellular processes.Binding, inhibition of the death inducing signaling complex(death-inducing SIGNAL CompleX PED/PEA-15to the molecularconfiguration of the special structure of the phase domain DED withother protein (such as: TNF-alpha and TRAIL and Fas, etc.) containingDED, DISC The formation thereby blocking the caspase cascadeactivation. Caspase via-intracellular role, i.e. mitochondrial pathways:Apoptosis-promoting substances, such as Bid, caspase8role may be asubstance released, i.e. cytochrome C (cytochrome, cytC), release cytCwith certain activated factor phase junction Caspase9and thus makedownstream Caspase3, Caspase6Caspase7are corresponding activationto apoptosis also occurred. Inhibitor of apoptosis protein PED/PEA-15expression and its role inGCA not been reported. In this study, by SP immunohistochemicalstaining and Western blot assay cardia adenocarcinoma tissue, cancertissue and the cardia and apoptosis in benign tissue inhibition of proteinPED/PEA-15expression, analysis and cardia adenocarcinomaclinicopathological relationship characterized by its long-term survivalafter relationship, evaluation of apoptosis inhibitory protein cardiaadenocarcinoma diagnosis and postoperative survival.Method: by using immunohistochemical SP method analysis ofhuman gastric cardia adenocarcinoma tumor tissue and pathology fornegative paraneoplastic organizations apoptosis inhibitory proteinPED/PEA-15in positive expression level of the same period cardiabenign lesions as control group. Fourth Hospital of Hebei Medicalspecimens taken from January2005to December cardia adenocarcinomapatients specimens of64cases, no preoperative line chemotherapy andhad complete follow-up records. Male35cases,29females between theages of45-75years old, with an average age of58.6years. According tothe degree of differentiation: poorly21cases, moderately differentiated43cases; cardia adenocarcinoma tumor infiltration depth: T1in17casesT211cases, T330T46cases; lymph node metastasis in39cases withoutlymph node metastasis25cases. According to the number of lymph nodemetastasis:0of25cases, one of20cases.≥19cases. Gastric CancerTNM (2010) staging standard: Ⅰa-b17cases, Ⅱa-b28cases, Ⅲ a-c19cases; follow-up results of the survival time (<20months,20months-40months,>40months) As the research stage. Cardia in patients withbenign lesions samples30were used as the control group. In addition,through another way, namely by Western blot specimen collection:specimens taken from the Fourth Hospital of Hebei Medical University,January2011-June cardia adenocarcinoma patients specimens of34cases,no preoperative line chemotherapy and have a more complete Thefollow-up record.26males,8females patients, between the ages of50-75 years old, with an average age of60.8years. According to the depth oftumor invasion: superficial infiltration of a group of10cases,24cases ofdeep infiltration group. According to the degree of differentiation: lowdifferentiated,23cases, moderately differentiated11cases; withoutlymph node metastasis in11cases,23cases of lymph node metastasis.Results:1Immunohistochemical detection of apoptosis to inhibit proteinPED/PEA-15cardia adenocarcinoma, cancer tissue and normal tissueexpression: expression of apoptosis to inhibit protein PED/PEA-15cardiaadenocarcinoma which positive (to+++positive) expressed as75%(48/64), negative (to-negative) expression in25%(16/64); expression incancer tissue, including positive expression56.3%(36/64), negativeexpression was43.7%(28/64); expression of cardia benign lesions,16.7%(5/30) positive expression, negative expression was83.3%(25/30).Statistical analysis showed that the three expression are different, and thedifference was statistically significant (P <0.05).2inhibitor of apoptosis protein PED/PEA-15cardia adenocarcino-ma expression and survival of relations: apoptosis to inhibit proteinPED/PEA-15in short-term survival group showed mostly highexpression in the long-term survival group Duocheng low expression, andthe inhibitor of apoptosis protein PED/PEA-15expression in eachsurvival group there is a significant difference (P <0.05).3immunohistochemistry apoptosis inhibitory protein PED/PEA-15cardia adenocarcinoma expression with clinical pathological features ofthe relationship: the cardia adenocarcinoma patients age, gender, and theinhibitor of apoptosis protein PED/PEA-15expression has nothing to do(all P>0.05); cardia adenocarcinoma cell differentiation, depth ofinvasion, lymph node metastasis, lymph node metastasis number andTNM stage inhibitor of apoptosis protein closely related to PED/PEA-15expression (all P <0.05).4Inhibitor of apoptosis protein PED/PEA-15expression cardia adenocarcinoma postoperative cumulative survival rate relationship:cardia adenocarcinoma patients with long-term survival and apoptosisinhibitory protein PED/PEA-15expression was negatively correlated(both P <0.05), apoptosis inhibit protein PED/PEA-15express the higher,the worse the long-term survival.5Western blot detection of apoptosis suppression the protein PED/PEA-15expression in each tissue in cancer tissues (2.189±0.367) wassignificantly higher than the adjacent tissues (1.321±0.249) and cardiacbenign mucosa (0.559±0.162)(P <0.01), cancer tissue was significantlyhigher than the cardia benign lesions mucosa (P <0.01).6Western blot detected in poorly differentiated group (2.343±0.698)was significantly higher than that of well-differentiated group (1.782±0.186), and between the two there is a statistically significant (P <0.05);deep infiltration group (2.273±0.789) significantly higher than the theshallow infiltration group (1.565±0.343) significant but with lymphnode metastasis (P <0.05);, the patient’s age and regardless of gender (P>0.05) between the two.Conclusions:1Immunohistochemical detection of apoptosis suppression ofprotein PED/PEA-15cardia adenocarcinoma and paraneoplasticorganizations expression levels significantly higher than the cardia benignlesions, apoptosis inhibitory protein PED/PEA-15expression levelscardia lesions.2Western Blot apoptosis inhibition of protein PED/PEA-15cardiaadenocarcinoma and paraneoplastic organizations expression level wassignificantly higher than the cardia benign lesions, apoptosis inhibitoryprotein expression levels and cardiac lesions PED/PEA-15nature related.3Immunohistochemical detection of apoptosis inhibition regardlessof the age and sex of the expression of the protein PED/PEA-15cardiaadenocarcinoma patients; closely related to the degree of tumordifferentiation, invasion depth and TNM staging.4、 Western Blot apoptosis inhibition regardless of the age and sex of the expression of theprotein PED/PEA-15cardia adenocarcinoma patients; is closely relatedwith the degree of tumor differentiation, invasion depth.5the inhibitor of apoptosis protein PED/PEA-15cardia adenocarcin-oma patients survival period was negatively related. The long termsurvival of patients with gastric cardia adenocarcinoma apoptosisinhibitory protein PED/PEA-15expression lower cumulative survival rate.Shorter long-term survival of patients with gastric cardia adenocarcinomaapoptosis inhibitory protein PED/PEA-15expression higher cumulativesurvival rate. The inhibitor of apoptosis proteins PED/PEA-15can beused as an important indicator to determine the prognosis of gastric cardiaadenocarcinoma.6the inhibitor of apoptosis protein PED/PEA-15cardia adenocarcin-oma plays an important role in the development of the inhibitor ofapoptosis protein PED/PEA-15GCA new target for targeted therapytheory reference.