The Study Of The Spleen Deficiency Syndrome Of Rheumatoid Arthritis From Fecal Supernatant Metabonomics And Genomics

Objective:To study the spleen deficiency syndrome of rheumatoid arthritis from fecal supernatant metabonomics and Genomics of traditional Chinese medicine. We want to build the metabolomics platform based on nuclear magnetic resonance NMR RA stool supernatant,analize the components and trends of RA patients stool supernatant of metabolites; to research spleen deficiency syndrome RA genomics, depict the expression profiles differences to find biomarkers indicative of the development of RA spleen Deficiency Syndrome, TCM Syndrome objective and standardized material base.Method:In the Metabonomics part, we specimens from patients and normal human feces, broken by ultrasound extraction after centrifugal, supernatant, in the NMR sample tube for NOESY spectrum acquisition. Orthogonal partial least squares discriminant analysis using SIMCA-P software (O-PLS-DA), as a metabolite information mining. Gene chip part of the extraction of deficiency of spleen vein blood of patients with RA, extraction of total RNA in blood, RNA samples were sent to the quality identification of Beijing Boao Biological Technology Company for microarray hybridization analysis, using the chip for the Agilent of human4*44K V2gene chip.The results:Spleen deficiency syndrome of RA patients and normal people can be distinguished, there is obvious difference between two groups of fecal metabolites. Compared with normal people, patients of RA with butyrate, propionate significantly down-regulation, glutamate, taurine, glucose, fumaric acid, tryptophan, succinic acid significantly increased. Compared with the non spleen deficiency syndrome, spleen deficiency of phenylalanine, leucine significantly up regulation of RA, glycine, propionic acid significantly reduced.According to the results of gene microarray, we found that in spleen deficiency of RA and healthy people there were633differentially expressed genes. The up expression gene are201, and down expression genes are432, respectively by the analysis of GO and pathway, they were found to be involved in25GO and37pathway, mainly related to inflammation, infection, protein metabolism, neuroendocrine immune network.Conclusion:There is a difference in the metabolites of The Spleen-deficiency syndrome of RA and expression of genomic background, which can be used as a reference of clinical research and syndrome essence.